羟基磷灰石硅胶复合材料的细胞毒性检测  

Cytotoxicity Study of Hydroxyapatite-silicone Composite

作　　者：吴国平[1] 杨锴[1] 滕利[1] 张芾男[1] 卢建建[1] 高寿松[1] 于洋[1] 

机构地区：[1]中国医学科学院中国协和医科大学整形外科医院创伤修复美容中心,100041

出　　处：《整形再造外科杂志》2005年第4期228-232,共5页

摘　　要：目的研究和开发羟基磷灰石硅胶复合材料,并对生产的4种组织代用品的细胞毒性进行了研究。方法将4种组织代用品的浸提液与宫颈癌HeLa细胞及淋巴母细胞AHH-1细胞接触2、3、5、6天后,用酶联免疫仪分别在492nm和570nm波长下测定各孔光吸收值,计算细胞相对增值率,用六级毒性分类法评级,并进行形态学观察。结果培养期4种材料浸提液培养的细胞均大量增殖,形态正常,毒性级为0～1级。结论羟基磷灰石硅胶复合材料具有良好的细胞生物相容性。Objective To study the cytotoxicity of four kinds of tissue substitutes and to develop a new hydroxyapaite-silicone composite as tissue substitute.Methods After the infusion of the four kinds of tissue substitutes was mixed with HeLa and AHH-1 cells for 2,3,5,6d,the relative cell proliferative rate was tested by ELISA.The cytotoxicity was graded by six-grade method.Cell morphological study was also performed.Results The cells in all the four substitutes infusion were all proliferated greatly with a normal form.The cytotoxicity was graded as 0～I.Conclusion Four kinds of tissue substitute all have good biocompatibility.

关 键 词：羟基磷灰石 硅胶 生物医学工程 细胞毒性 

分 类 号：R318.08[医药卫生—生物医学工程]