麦冬对甲基磺酸甲酯诱发的小鼠精子非程序DNA合成的抑制作用  

Inhibitory effect of Radix ophiopogonis on unscheduled DNA synthesis induced by methyl methanesulfonate in spermatogod of mice

作　　者：朱玉琢[1] 庞慧民[1] 刘念稚[2] 

机构地区：[1]吉林大学基础医学院医学遗传学教研室 [2]吉林省人民医院

出　　处：《吉林大学学报（医学版）》2002年第5期461-462,共2页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技发展计划项目 (2 0 0 10 5 2 9)

摘　　要：目的 :观察中草药麦冬对雄性小鼠生殖细胞遗传物质损伤的防护作用。方法 :采用雄性小鼠生殖细胞非程序 DNA合成实验。结果 :麦冬各剂量组诱导的非程序 DNA合成与正常对照组比较差异无显著性 ( P>0 .0 5 ) ;6.8～ 1 3.6g· kg-1剂量 ,麦冬对甲基磺酸甲酯所诱导的非程序 DNA合成有明显抑制作用 ( P<0 .0 1 ) ,并且随着麦冬浓度的增加 ,其抑制作用逐渐增强 ,超过一定剂量 ,其抑制作用不再增强。结论Objective:To observe the preventive effect of Radix ophiopogonis on damage of genetic materials in male mouse reproductive cells.Methods:Unscheduled DNA synthesis UDS test in male mouse reperoductive cells was used. Results:There was no significant difference ( P >0.05) while UDS induced by Radix ophiopogonis of various dose comparing with normal control. Nevertheless,Radix ophiopogonis in dose ranged from 6.8 g·kg -1 to 13.6 g·kg -1 ,inhibited strikingly UDS induced by MMS ( P <0.01), and the inhibitory effect was enhanced with the incresse of the concentration. However the effect was not changed when the dosage reached a certain concentration . Conclusion: Radix ophiopogonis shows a protective effect, namely antimutagenesis,on injury of genetic materials in reproductive cells of male mouse

关 键 词：麦冬 甲基磺酸甲酯 小鼠 精子 非程序DNA合成 抑制作用 

分 类 号：R282[医药卫生—中药学]