HPLC法测定人血浆中头孢克洛浓度  

Determination of Cefaclor in Human Plasma by HPLC

作　　者：张静[1] 赵悦[1] 刘广余[1] 李健华[2] 江翊国[3] 

机构地区：[1]南京医科大学第一附属医院,南京210029 [2]徐州医学院,徐州221002 [3]苏州市儿童医院,苏州215003

出　　处：《江苏药学与临床研究》2002年第3期11-13,共3页Jiangsu Pharmacertical and Clinical Research

摘　　要：目的:采用高效液相色谱法测定人血浆中头孢克洛浓度。方法:以头孢拉定为内标,HPLC法测定。结果:头孢克洛的线性范围为0.25～30μg/ml,回归方程为y=0.1105+0.0817x,r=0.9995,最低检测浓度为0.125μg/ml,日内RSD为1.61％～3.54％,日间RSD为2.15％～5.08％。结论:主要药代动力学参数与文献报道相似。AIM A HPLC method was established for the determination of cefaclor in human plasma. METHOD Cefradine was used as internal standard. HPLC method was used. RESULTS The range of calibration was 0. 25-30 ?g/ml(y=0. 1105 + 0. 0817x,r = 0. 9995). The minimal detectable drug concentration in plasma was 0. 125 ng/ml. RSD whinin-day was 1. 61% - 3. 54%. RSD day-today was 2. 15%-5. 08%. CONCLUTION The pharmacokinetic parameters are similar to those reported in foreign journals.

关 键 词：头孢克洛 高效液相色谱 血药浓度 药代动力学 

分 类 号：R928.11[医药卫生—药学] R969.1