核酸扩增技术在诊断柯萨奇B组病毒性心肌炎中的应用  

A RT-PCR Assay for Diagnosing Viral Myocarditis Caused by Coxsackie B and Its Application

作　　者：杜琼[1] 蔡力[1] 李英[1] 王勇[2] 杨明清[1] 邓君[1] 林婴[1] 

机构地区：[1]四川省人民医院,四川成都610072 [2]川北医学院附属医院,四川南充637000

出　　处：《川北医学院学报》2002年第3期5-7,共3页Journal of North Sichuan Medical College

摘　　要：目的　寻找一种简便、快速、准确、科学检测CVB的方法 ,同时筛选最佳检测标本。方法选择实验组 95例 (病毒性心肌炎病人 ) ,对照组 30例 (健康体检者 )分别采集两次四种不同的标本 ,利用核酸扩增技术检测CVB -RNA。结果四种不同标本 ,白细胞的检出率最高 (5 4 .7% ) ,咽拭子次之 (42 .1% ) ,血清为 (16 .7% ) ,尿液为 (4.2 % )。结论采集病毒性心肌炎病人的白细胞、咽拭子 ,应用核酸扩增技术RT -PCR进行CVB -RNA的检测 ,是诊断由CVB引起的病毒性心肌炎的一种早期、准确、灵敏。Objective To develop a simple ,sensitive and reliable assay for detection of CVB and screen which kinds of sample is the best.Methods A RT-PCR assay was utilized to detect the level of CVB in samples of 95 patients with viral myocarditis,30 with healthy controls.Samples were in white blood cell,throat swabs,serum and urine respectively.Results The defecrate of samples 54.7% in white bloo cell, 42.1% in throat swabs, 16.7% in serum and 4.2% in urine, respectives Conclusion Measurement of CVB-RNA in white blood cell and throat swabs can be used for the early diagnosis of viral myocarditis.

关 键 词：核酸扩增技术 诊断 病毒性心肌炎 柯萨奇B组病毒 

分 类 号：R542.21[医药卫生—心血管疾病]