兔体内乌头碱代谢产物研究  

STUDY ON METABOLITES OF ACONITINE IN RABBIT URINE

作　　者：孙莹[1] 张宏桂[1] 史向国[2] 段明郁[1] 钟大放[2] 

机构地区：[1]吉林大学药学院天然药物化学教研室,吉林长春130021 [2]沈阳药科大学药物代谢与药物动力学实验室,辽宁沈阳110016

出　　处：《药学学报》2002年第10期781-783,共3页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目 (3 9870 874;3 993 0 180 ) .

摘　　要：目的　鉴定乌头碱在家兔尿中主要代谢产物。方法　ig给药 (5mg·kg- 1 )后 ,收集给药后♂家兔尿液 ,固相萃取柱富集、液相色谱 电喷雾离子阱质谱法分离鉴定乌头碱代谢物。结果　在给药后尿样中发现乌头碱原形和4种代谢物M1～M4 ,分别测得准分子离子峰 [M +H]+ 及其各级碎片离子。结论　M1～M4为乌头碱的 4个代谢物 ,推测分别为 16 O 去甲基乌头碱、乌头次碱、16 O 去甲基乌头次碱、乌头原碱。AIM To identify the main metabolites of aconitine in the urine of rabbits. METHODS After oral administration of aconitine (5 mg·kg -1 ), the urine of male rabbits was collected and extracted by solid phase extraction and analyzed by liquid chromatography ion trap mass spectrometry. RESULTS Aconitine and 4 metabolites were found in the rabbit urine. Their protonated molecular ions at m/z 632, m/z 604, m/z 590, m/z 500 and multistage fragment ions with neutral loss of 60 u, 32 u, 28 u and 18 u were monitored. Their relative concentration were M1>Aconitine>M4>M2>M3. CONCLUSION The metabolites M1～M4 were deduced as 16 O demethylaconitine, benzoylaconine, 16 O demethylbenzoylaconine and aconine, respectively.

关 键 词：乌头碱 代谢物 液相色谱-电喷雾离子阱质谱法 中毒机制 动物实验 

分 类 号：R285.1[医药卫生—中药学]