铁破锣皂苷O和P的结构及其药理活性  

STRUCTURES AND PHARMACOLOGICAL ACTIVITIES OF BEESIOSIDES O AND P

作　　者：鞠建华[1] 林耕[1] 杨峻山[1] 逯海燕[1] 马柄娜[2] 聂淑芹[3] 张侠[4] 

机构地区：[1]中国协和医科大学,中国医学科学院药用植物研究所,北京100094 [2]北京大学基础医学院免疫学系,北京100083 [3]中国中医研究院中药研究所药理室,北京100700 [4]北京军区总医院肿瘤科,北京100700

出　　处：《药学学报》2002年第10期788-792,共5页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助重点项目 (2 973 2 0 40 )

摘　　要：目的　研究我国特有药用植物铁破锣 [Bessiacalthaefolia (Maxim .)Ulhr.]根茎的化学成分。方法　利用各种色谱技术进行分离 ,根据化合物的光谱数据 (IR ,MS ,1 HNMR ,1 3CNMR ,2DNMR)和化学方法鉴定其结构 ,并对所得单体成分进行药理活性筛选。结果　从甘肃产铁破锣根茎的氯仿萃取物中分离得到 2个化合物 ,分别鉴定为 :(2 0S ,2 4S) 15α acetoxy 16 β ,2 4 ;2 0 ,2 4 diepoxy 9,19 cyclolanostane 3β ,2 5 diol 3 O β D xylopyranoside (I)和 (2 0S ,2 4R) 15α acetoxy 9,19 cyclolanostane 3β ,16 β ,2 0 ,2 4 ,2 5 pentaol 3 O β D xylopyranoside (II) ,分别命名为铁破锣皂苷O(beesiosideO)和铁破锣皂苷P(beesiosideP)。结论　I和II为新化合物 ,I有免疫抑制、抑制血管生成和抑制成骨细胞增殖活性。AIM To investigate the chemical constituents of the rhizomes of Beesia calthaefolia native to China in order to obtain a more comprehensive understanding of its effective components. METHODS Compounds were isolated by column chromatography with silica gel. Their structures were elucidated by spectral analysis and chemical evidence. Compounds identified were subjected to pharmacological evaluation. RESULTS Two novel compounds were isolated and identified as (20 S ,24 S ) 15α acetoxy 16β,24;20,24 diepoxy 9,19 cyclolanostane 3β,25 diol 3 O β D xylopyranoside (I) and (20 S ,24R) 15α acetoxy 9,19 cyclolanostane 3β,16β,20,24,25 pentaol 3 O β D xylopyranoside (II), named beesioside O and beesioside P. CONCLUSION Compounds I and II are new compounds. Compounds I exhibited immunosuppressive activity and could inhibit angiogenesis as well as inhibit the proliferation of osteoblast. Compound II displayed remarkable inhibition activity against calcium channel receptor.

关 键 词：铁破锣 铁破锣皂苷O 铁破锣皂苷P 药理活性 结构鉴定 

分 类 号：R282.710.5[医药卫生—中药学]