rhEPO结合高低氧对贫血荷瘤小鼠放射增敏的效应  

EFFECTS OF rhEPO COMBINING CARBOGE N PLUS LOW CONCENTRATION OXYGEN ON ENHANCED RADIOSENSITIVITY FOR TUMORS IN ANEMI C MICE

作　　者：陈卫东[1] 张桂琦[1] 安永恒[2] 

机构地区：[1]济宁市第一人民医院放疗中心,山东省济宁市272111 [2]青岛大学医学院附属医院肿瘤治疗研究中心

出　　处：《齐鲁医学杂志》2002年第4期286-287,289,共3页Medical Journal of Qilu

基　　金：青岛市自然科学基金资助项目 ( 1998- 2 4 )

摘　　要：①目的　观察促红细胞生成素 (rhEPO)结合高低氧对贫血荷瘤小鼠放射增敏的效应。②方法　昆明小鼠随机分为 3组 :A组加用rhEPO预防卡铂诱导的贫血 ,然后对小鼠荷瘤肢行高低氧放疗 ;B组用卡铂诱导小鼠贫血 ,对小鼠荷瘤肢行高低氧放疗 ;C组用卡铂诱导小鼠贫血 ,对小鼠荷瘤肢放疗。观察 3组放射效应的差别 ,最后各组均行肿瘤组织活检。③结果　从肿瘤种植后第 1 4天开始 ,A组肿瘤重新生长的速度与B ,C组比较明显延缓(T =0～ 3 ,P <0 .0 5) ;C组与B组比较 ,B组肿瘤细胞种植后第 1 8,2 2天重新生长速度较C组慢 (T =2 ,3 ,P <0 .0 5)。A组的肿瘤倍增时间较B组、C组长。病理切片显示A组肿瘤细胞坏死明显 ,仅有少数细胞残核 ,B组肿瘤细胞部分坏死 ,C组肿瘤细胞坏死不明显。④结论　rhEPO结合高低氧放疗对贫血荷瘤小鼠具有放射增敏的作用 。Objective\ To investigate the effects of re co mbinant human erythropoietin(rhEPO) combining carbogen plus low concentration ox ygen radiotherapy (CPLCOR)on enhanced radiosensitivity for tumors in anemic mice . \ Methods\ Kun Ming mice were randomly divided into three groups: Gr oups A, B and C. The mice in Group A were pretreated with rhEPO to prevent anemi a induced by carboplatin, and then the tumors were irradiated with CPLCOR. Anemi a of the mice in Group B and Group C was induced by carboplatin. The tumors in Group B were irradiated with CPLCOR; tumors in Group C were treated with irradia tion only. The differences of effects of irradiation were investigated and biops ies were done for all experimental mice. \ Results Fourteen days after tumor implantation, the regenerati on speed of the tumors in Group A was dramatically slowed compared with that in Group B and C ( T=0-3,P <0.05). A comparison between Group B and Group C showe d that the speed of regeneration of tumors in Group B, 18 and 22 days after impl antation of the tumors, was slower than that in Group C ( T=2,3,P <0.05). The volume doubling time of tumors in group A was longer than that of the tumors in Groups B and C. The histology indicated that necrosis of tumor cells was eviden t with few residual nuclear in Group A, partial necrosis in Group B but not obvi ous in Group C. Conclusion\ The rhEPO treatment combining CPLCOR increases tumo r radiosensitivity of anemic mice and it is advocated clinically. [

关 键 词：荷瘤小鼠 促红细胞生成素 贫血 癌症 高低氧放射疗法 

分 类 号：R556[医药卫生—血液循环系统疾病] R73－354[医药卫生—内科学]