Clara细胞分泌蛋白在实验性气道炎症中的表达  

Expression of Clara Cell Secretory Protein in Asthmatic Rat Model

作　　者：罗凤鸣[1] 王曾礼[1] 刘小菁[2] 刘春涛[1] 章小红[1] 王文志[1] 

机构地区：[1]四川大学华西医院呼吸内科,成都610041 [2]四川大学华西医院内科实验室

出　　处：《华西医科大学学报》2002年第4期511-514,共4页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金资助 (批准号 3 9770 3 40 )

摘　　要：目的　研究哮喘大鼠气道局部 Clara细胞分泌蛋白 (CCSP) m RNA的表达及作用。方法　用卵白蛋白致敏、激发建立大鼠哮喘模型 ,以逆转录聚合酶链反应、斑点免疫印迹及免疫组化方法分别检测大鼠激发哮喘后3、7和 14天肺组织 CCSP m RNA、支气管肺泡灌洗液 (BAL F)中 CCSP蛋白水平及细小支气管 Clara细胞比率。结果激发哮喘后大鼠 CCSP m RNA表达减少 (对照为 0 .6 5± 0 .0 5 ,激发 3天、1周及 2周后分别为 0 .5 7± 0 .0 5、0 .5 1±0 .0 4及 0 .5 0± 0 .0 3,P均 <0 .0 1) ,BAL F中 CCSP蛋白含量降低 (对照为 5 9.6 7± 4 .72 ,激发 3天、1周及 2周后分别为 4 9.33± 4 .6 3、4 4 .0 0± 4 .6 3、4 3.5 0± 2 .5 9,P均 <0 .0 1) ,Clara细胞比率下降 (P<0 .0 1或 0 .0 5 )。结论　哮喘大鼠 CCSP m RNA表达减少 ,CCSP水平降低 ,可能通过对磷脂酶Objective To investigate the expression of Clara cell secretory protein (CCSP) mRAN in rats with asthma. Methods The asthmatic rat model was established using aerosolized ovalbumin (OVA). The CCSP mRNA in lung tissue, CCSP in bronchial alveolar lavage fluid (BALF), and ratio of Clara cell in bronchiole were determined by RT PCR, dot immuno blotting, and immunohistochemistry, respectively. Results Progressive decline of CCSP mRNA in lung tissue(0.65±0.05, 0.57±0.05,0.51+0.04 and 0.50+0.03 in control,challenged 3 days,1 week, and 2 weeks, respectively) and CCSP level in BALF 〔59.67±4.72, 49.33±4.63, 44.00±4.63 and 43.50±2.59 in control,challenged 3 days,1 week and 2 weeks, respectively ( P <0.01)〕 were found. The ratio of Clara cell in the airway epithalamia also reduced 3, 7 and 14 days after challenge ( P <0.01 or 0.05). Conclusion The CCSP mRNA and its transcribed products may play roles in asthmatic airway inflammation.

关 键 词：支气管哮喘 CLARA细胞分泌蛋白 表达 

分 类 号：R562.25[医药卫生—呼吸系统]