中风脑得平冲剂防治大鼠脑缺血再灌注损伤的实验研究  

Effect of Zhongfeng Nao De Ping on delayed neuronal death in hippocampus following cerebral ischemia/reperfusion in rats

作　　者：李克玲[1] 黄启福[1] 邹丽琰[1] 蒋玉凤[1] 严京[1] 贾旭[1] 李伯光[1] 成立华[2] 

机构地区：[1]北京中医药大学病理学教研室,北京100029 [2]北京中医药大学光谱室,北京100029

出　　处：《中国病理生理杂志》2002年第9期1058-1061,共4页Chinese Journal of Pathophysiology

基　　金：国家"八五"攻关课题科研基金资助项目 (No .85 - 919- 0 2 - 0 1)

摘　　要：目的与方法 :以雄性Wistar大鼠双侧颈总动脉缺血再灌注模型 ,观察中风脑得平冲剂对脑组织含水量 ,海马Ca2 + 、兴奋性氨基酸含量及形态学变化的影响 ,以探讨药物的作用机制。结果与结论 :大鼠脑缺血再灌注后 ,有明显脑水肿及海马神经元损伤 ,脑组织含水量 ,Ca2 + 及兴奋性氨基酸的含量明显升高 ,中风脑得平冲剂表现出不同程度的抑制上述指标异常升高的作用 ,从而保护神经元减轻损伤。AIM: To investigate the effect of Zhongfeng Nao De Ping, a traditional chinese medicine, on the cerebral ischemia/reperfusion injury in rats. METHODS: Delayed neuronal death (DND) model was established by bilateral carotid arteries occlusion for l0 min followed by 1 day and 7 days reperfusion. Effects of sodium glutamate, ketamine and Zhongfeng Nao De Ping on DND in the hippocampus was examined by counting neuronal density in CAl Sector, and the contents of Ca 2+ and amino acids in the hippocampus were also measured. RESULTS: The contents of the glutamate and aspartate in the hippocampus were increased significantly at l0 min after transient cerebral ischemia. Sodium glutamate markedly decreased the neuronal density in hippocampal CAl sector, while ketamine and Zhongfeng Nao De Ping increased it significantly in the same sector. The level of Ca 2+ increased on day 1, day 7 after the reperfusion, while Zhongfeng Nao De Ping inhibited the increase in Ca 2+ concentration in hippocampus. CONCLUSION: These results suggested that Zhongfeng Nao De Ping has an effective protection against DND in hippocampus following cerebral ischemia/reperfusion in rats.

关 键 词：中风脑得平冲剂 防治 大鼠 脑缺血再灌注损伤 实验研究 

分 类 号：R277.733[医药卫生—中医学] R285.5