慢性庆大霉素损伤后豚鼠前庭上皮细胞的凋亡和再生观察  

Apoptosis and Proliferation Following Chronic Gentamicin Toxicity in Guinea Pig Vestibular Sensory Epithelium

作　　者：马晓莉[1] 黄维国[2] 王锦玲[2] 姜鸿彦[2] 刘顺利[2] 邱建华[2] 

机构地区：[1]空军总医院耳鼻咽喉科,北京100036 [2]第四军医大学西京医院耳鼻咽喉科

出　　处：《听力学及言语疾病杂志》2002年第4期241-243,I001,共4页Journal of Audiology and Speech Pathology

基　　金：国家自然科学基金资助项目 (3 9870 788)

摘　　要：目的　观察慢性庆大霉素中毒后豚鼠前庭上皮的损伤和修复过程 ,探讨前庭毛细胞凋亡和再生的关系。方法　采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记 (terminaldeoxyuncleotidyltransferase -mediateddeoxyuridinetriphosphatenickendlabeling ,TUNEL)法 ,观察前庭感觉上皮细胞的凋亡 ,采用 5’ -溴 - 2 -脱氧尿苷(bromodeoxyuridine,Brdu)免疫细胞化学法观察前庭上皮的再生过程。 结果　对照组没有观察到TUNEL和Brdu阳性细胞。停止庆大霉素注射 1天至 3周均可观察到TUNEL阳性细胞 ,但 1天组阳性细胞数量最多 ,3周仍有阳性细胞存在 ,阳性细胞主要位于毛细胞层。停止庆大霉素注射后 1天组支持细胞层和毛细胞层均有Brdu标记阳性细胞 ,3d组支持细胞层阳性细胞显著增多 ,1周组毛细胞层也出现较多阳性细胞 ,2周组Brdu标记阳性细胞主要位于毛细胞层 ,3周仍可观察到Brdu标记阳性细胞。结论　慢性庆大霉素耳中毒后豚鼠前庭上皮细胞凋亡增加 ,凋亡与增殖伴随存在 ,前庭毛细胞的损伤可能是其增殖的触发因素。增殖过程表现为支持细胞增生向毛细胞增生移行的趋势 ,支持细胞增殖并转化成毛细胞可能是前庭上皮细胞损伤修复机理之一。Objective To study the process of injury and repair of vestibular epithelium of guinea pigs after chronic toxicosis of gentamicin, and to explore the relationship between the vestibular hair cell apoptosis and regeneration.Methods The apoptosis of vestibular epithelium was detected by terminal deoxyuncleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling(TUNEL)method and the cell regeneration by anti-bromodeoxyuridine (Brdu) immunohistochemical method.Results There were no TUNEL or Brdu positive cells in control group. From 1d to 3 wk after gentamicin treatment the TUNEL positive cells were detected, a large number of positive cells was seen in 1 d group and then the positive cells were decreased. Positive cells still could be seen in 3 wk group. The positive cells predominantly located in hair cell layer. 1 d after gentamicin treatment, the Brdu positive cells could be seen either in supporting cell layer or in hair cell layer. In 3 d group the positive cells in supporting cell layer were increased. In 1 wk group some positive cells in hair cell layer were found. In 2 wk group the Brdu positive cells mainly located in hair cell layer and the positive staining lasted until 3 wk after gentamicin treatment.Conclusion After chronic toxicosis of gentamicin, the apoptosis of guinea pig vestibular epithelium was increased. Cell proliferation and apoptosis accompanied with each other, and the apoptosis might be a trigger factor of proliferation. There was a transition trend of supporting cell proliferation to hair cell proliferation. The proliferation of supporting cell and its transform into hair cell might be one of the repair mechanism of vestibular hair cell after injury.

关 键 词：前庭感觉上皮 细胞凋亡 庆大霉素 前庭毛细胞损伤 耳毒性 

分 类 号：R764.43[医药卫生—耳鼻咽喉科] R978.12[医药卫生—临床医学]