模拟失重大鼠动脉血管紧张素受体基因表达的研究  

A Study on Gene Expression of Angiotensin Receptors in Arteries from Tail-suspended Rats.

作　　者：张乐宁[1] 孟庆军[1] 张立藩[1] 马进[1] 

机构地区：[1]第四军医大学航空航天生理学教研室,陕西西安710032

出　　处：《航天医学与医学工程》2002年第5期343-346,共4页Space Medicine & Medical Engineering

基　　金：国家自然科学基金资助项目 (3 0 1710 3 2 ) ;全军医药卫生科研基金资助项目 (0 1Q114 )

摘　　要：目的探讨模拟失重下大鼠不同部位动脉血管紧张素受体mRNA表达是否发生变化。方法大鼠被随机分为模拟失重组 (SUS)与同步对照组 (CON) ,采用尾部悬吊大鼠模型模拟失重影响。用逆转录 -聚合酶链式反应 (RT PCR)技术观察比较模拟失重下大鼠不同部位动脉血管紧张素两型受体基因表达的变化。结果大鼠颈总动脉 ,腹主动脉及股动脉组织均有血管紧张素Ⅱ (AngⅡ )的AT1a,AT1b及AT2 受体mRNA的表达 ;而基底动脉组织则只有AT1a和AT1bmRNA的表达。模拟失重 4周大鼠颈总动脉、腹主动脉、股动脉及基底动脉组织AngⅡ的AT1a、AT1b和AT2 受体mRNA的表达 ,较对照大鼠均无显著变化。Objective To investigate whether simulated microgravtiy can result in alterations of mRNA expression of angiotensin receptors in rat arteries. Method Rats were divided into simulated microgravity group and synchronous control group. Simulated microgravity rats were subjected to tail suspension for 4 wk to simulate the effect of weightlessness. Result Results from RT PCR demonstrated that mRNAs of angiotensinⅡreceptors, AT 1a ,AT 1b and AT 2 were expressed in carotid arteries, abdominal aorta and femoral arteries, whereas only mRNAs of AT 1a and AT 1b were expressed in basilar arteries. There were no significant differences between the simulated microgravtiy and control rats in the expression of mRNAs of AT 1a ,AT 1b and AT 2 in these arteries. Conclusion AngiotensinⅡreceptors may play no regulatory role in the occurrence of the differentiated vascular adaptation during simulated microgravity.

关 键 词：模拟失重 血管紧张素受体 基因表达 尾部悬吊 逆转录聚合酶链反应 肾素-血管紧张素系统 血管紧张素Ⅱ 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]