5Hz和20Hz磁场对中脑神经干细胞分化的影响  

Effects of Different Frequency Electromagnetic Fields on the Differentiation of Midbrain Neural Stem Cells.

作　　者：李怡[1] 赵仑[2] 邢萱[3] 娄淑杰[1] 何成[1] 路长林[1] 

机构地区：[1]第二军医大学神经生物学教研室,上海200433 [2]航天医学工程研究所,北京100094 [3]第二军医大学数理教研室,上海200433

出　　处：《航天医学与医学工程》2002年第5期374-376,共3页Space Medicine & Medical Engineering

摘　　要：目的观察 5Hz和 2 0Hz磁场 (EMF)对新生大鼠中脑神经干细胞神经元向分化的影响。方法神经干细胞分别置于 5Hz和 2 0Hz正弦交变磁场 ( 8mT)中诱导分化 ,每天上午、下午各 1次 ,每次 1 5min ,分别作用 1d、5d或 1 0d ,同时设立相应的对照组。利用神经元特异性标记物MAP2 抗体进行免疫荧光染色 ,计数MAP2 阳性细胞的百分比。结果 5Hz和 2 0Hz交变磁场干预 1d ,均出现对NSC分化方向的显著影响 ,即促进NSC神经元向的分化 ;2 0Hz电磁场随干预时程的延长 ,NSC分化为神经元的比例逐渐增高 ,以 1 0d组神经元比例最高 ;5Hz磁场干预 5d时神经元比例最高 ,1 0d时有所下降 ,但仍高于对照组 ;2 0Hz磁场效应在 1 0d时显著大于 5Hz磁场。结论 5Hz和 2 0Hz电磁场以不同的效应模式促进NSC神经元向的分化 ,电磁场可以成为NSC定向分化研究的重要手段之一。Objective To examine the effect of electromagnetic fields (EMF) (20 Hz, 8 mT; 5 Hz, 8 mT) on the neuron orientated differentiation of neural stem cells (NSCs) from midbrains of new born rats. Method Differentiated NSCs were exposed to EMF for 2×15 min per day lasting for 1 d, 5 d, or 10 d. The sham exposure controls were correspondingly established. Cells were fixed and processed for immunofluorescent staining using the antibody against neuron specific marker MAP 2, then the percentage of MAP 2 + cells was calculated. Result The two EMFs promoted the differentiation of a neuronal fate in different ways. Both of them came into effect even after 1 d exposure. When cells exposed to the 20 Hz EMF, the percentage of neuron orientated cells gradually increased with longer term exposure and the most significant effect appeared in 10 d group while that happened in 5 d group under the condition of 5 Hz EMF. The effect contrasted horizontally, significant differences between the two EMFs were observed only at 10 d groups, 20 Hz EMF having more favorable effect than 5 Hz EMF. Conclusion 20 Hz and 5 Hz EMF could promote the differentiation of midbrain NSCs to a neuronal phenotype in different ways, suggesting that the physical induction might be another strategy to manipulate the differentiation of NSCs.

关 键 词：电磁场 神经干细胞 细胞分化 免疫荧光技术 恒磁场 交变磁场 

分 类 号：R338.8[医药卫生—人体生理学] R392－33[医药卫生—基础医学]