鸭胚胎血液中PGCs数量及其外源DNA的体内转染  

Observations of the Primordial Germ Cells in Embryonic Blood and in Vivo DNA Transfer into the Primordial Germ Cells in Sheldrake

作　　者：叶荣忠[1] 张彦定[1] 高建民[1] 郭小玲[2] 

机构地区：[1]福建师范大学生物工程学院,福建福州350007 [2]厦门大学海洋与环境学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2002年第5期623-627,共5页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金 (39770 377);福建省自然科学基金资助项目

摘　　要：抽取了麻鸭和骡鸭胚胎发育第 14～ 2 0期的血液 ,制作血涂片 ,PAS染色 .观察血液中的原生殖细胞的含量 ,并对各个时期血液中原生殖细胞的含量进行了统计 ,最高含量都出现在第 16期 ,分别为 8.975 2 %和 2 .1871% .用InvivoGeneDeliverySystem和 pEGFP N对PGCs进行了外源DNA的体内转染 .结果在 6只胚胎中的The amount of the primordial germ cells in embryonic blood from stages 14 through stages 20 in the Sheldrake's and mule-duck's embryos were counted by PAS reaction. The results showed that the highest concentration of primordial germ cells was at stages 16 both in Sheldrake and mule-duck, and the concentration was 8.975 2% for Sheldrake and 2.187 1% for mule-duck. Meanwhile, GFP report gene transfer into Sheldrake's blood PGCs in vivo was processed. The results showed that the expressions of GFP were found in 3 embryos.

关 键 词：胚胎血液 PGCS 外源DNA 体内转染 麻鸭 骡鸭 原生殖细胞 胚胎发育 细胞数量 基因转移 

分 类 号：S834[农业科学—畜牧学] Q954.4[农业科学—畜牧兽医]