NADH-细胞色素b_5还原酶在甲状腺过氧化氢生物合成中的作用  

Relation of NADH-cytochrome b_5 Reductase in Hydrogen Peroxide Biosynthesis of Thyroidea

作　　者：黄国良[1] 张翼[1] 张更[2] 高妍[3] 朱进伟[2] 

机构地区：[1]福建医科大学附属协和医院,福建省内分泌研究所,福州350001 [2]福建医科大学基础医学院,福州350004 [3]北京大学第一医院内分泌科,北京100034

出　　处：《福建医科大学学报》2002年第3期250-253,共4页Journal of Fujian Medical University

基　　金：福建省医学重点学科基金 (19982 2 3 );福建省科技厅科研基金资助项目 (K2 0 0 62 )

摘　　要：目的　探讨 NADH-细胞色素 b5还原酶 (b5R)在甲状腺过氧化氢生物合成中作用。　方法　应用高香草酸荧光分析技术及 NADH-高铁氰化钾还原酶法 ,对正常和格雷夫斯病甲状腺过氧化氢 (H2 O2 )和 b5R进行测定 ,同时观察 b5R抑制剂对 H2 O2 生成和蛋白结合碘形成的影响。　结果　格雷夫斯病甲状腺 b5R活性和 H2 O2 水平均明显高于正常 ,而 H2 O2 酶活性在两者间差异无显著性。加 b5R抑制剂对氯汞苯甲酸抑制 b5R活性 ,格雷夫斯病和正常甲状腺 b5R活性降低近 85 % ,同时 H2 O2 降低近 5 0 % ,蛋白结合碘形成减少近 5 2 %。 b5R活性和 H2 O2 水平两者呈显著正相关。　结论　 b5R参与甲状腺内 H2 O2 的生物合成 ,是甲状腺内产生 H2 O2 的重要酶系。Objective\ To investigate the role of NADH\|cytochrome b\-5 reductase(b\-5R) in biosynthesis of hydrogen peroxide in thyroid.\ Methods\ The activity of b\-5R and the levels of hydrogen peroxide in the thyroid of patients with Graves' disease(GD) and normal controls were measured with potassium ferricyanide as substrate and with the homovanillic acid fluorescence assay.\ Results\ The activity of b\-5R and the level of H\-2O\-2 in GD thyroid were definitely higher than those in normal control, but the activity of catalase was not different between GD and normal thyroid.After addition of p\|chloromercuribenzoate, a b\-5R inhibitor, the activity of b\-5R in GD and normal thyroid decreased by 85%, the level of H\-2O\-2 decreased by 50%, protein\|bound iodine formation decreased by 52%.\ There is a positive correlation between the level of H\-2O\-2 and the activity of b\-5R.\ Conclusion\ b\-5R participates in thyroid hydrogen peroxide biosynthesis and which is an important emzyme in the production of H\-2O\-2.\;

关 键 词：NADH-细胞色素B5还原酶 过氧化氢 格雷夫斯病 高香草酸荧光分析技术 NADH-高铁氰化钾还原酶法 

分 类 号：R581.103[医药卫生—内分泌]