GnRH类似物对培养的大鼠胃平滑肌细胞增殖的影响  

EFFECTS OF GnRH ANALOG ON THE PROLIFERATION OF CULTURED GASTRIC SMOOTH MUSCLE CELLS OF RATS

作　　者：陈蕾[1] 黄威权[1] 孙绪德[2] 赵晶[3] 吕葆真[1] 蒲若蕾[1] 

机构地区：[1]第四军医大学组织胚胎学教研室,西安710033 [2]唐都医院麻醉科,西安710038 [3]生物化学教研室,西安710033

出　　处：《解剖学报》2002年第5期505-510,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 (3 9770 3 88)

摘　　要：目的　观察促性腺激素释放激素 (gonadotropin releasinghormone,GnRH)类似物阿拉瑞林 (alarelin)对培养的大鼠胃平滑肌细胞增殖的影响。　方法　应用离体培养的SD大鼠胃平滑肌细胞 (gastricsmoothmusclecell,GSMC) ,采用四唑盐 (MTT)比色法实验 ,3H 胸腺嘧啶核苷酸 (3H TdR)参入、免疫荧光化学检测增殖细胞核抗原 (pro liferatingcellnuclearantigen ,PCNA)表达的平均荧光值和流式细胞仪技术 ,观察阿拉瑞林对GSMC的增殖、DNA合成和细胞周期的影响。　结果　当加入终浓度为 10 - 9、10 - 7、10 - 5mol L的阿拉瑞林 2 4h后 ,与未用药的对照组相比 ,发现其MTT吸光度 (A值 )逐渐降低 (P <0 0 5 ) ;PCNA表达的平均荧光值逐渐减弱 (P <0 0 5 ) ;GSMC的3H TdR参入量依次减少 (P <0 0 5 ) ,且药物浓度越大 ,此三者下降越明显。在细胞周期中 ,与对照组相比 ,G1 期细胞所占比例明显增加 (P <0 0 5 ) ;S期细胞所占比例明显减少 ,且随药物浓度的增加而逐渐减少 (P <0 0 5 )。　结论　GnRH类似物可明显抑制大鼠GSMC的增殖作用 。Objective To investigate the effects of gonadotropin releasing hormone(GnRH) analog (Alarelin) on the proliferation, the DNA synthesis and cell cycle of rat gastric smooth muscle cells(GSMC). Methods Techniques of cell culture, OD value of MTT test, measure of 3H TdR incorporation, average fluorescent values of proliferating cell nuclear antigen (PCNA) and flow cytometric DNA analysis were used. Results When alarelin(10 -9 ,10 -7 ,10 -5 ?mol/L) were administered into the medium and incubated for 24?h, OD value of MTT, 3H TdR incorporation and average fluorescent values of PCNA all decreased significantly as compared with control group ( P <0 05). Flow cytometric DNA analysis revealed that alarelin could significantly enhance ratio of G 1 phase and decrease ratio of S phase of GSMC of rats ( P <0 05). Conclusion The results show that GnRH analog can directly inhibit proliferation and DNA synthesis of rat GSMCs through themself GnRH receptors.

关 键 词：GNRH类似物 大鼠 胃平滑肌 细胞增殖 影响 

分 类 号：R977.1[医药卫生—药品] R965[医药卫生—药学]