人前磨牙牙髓牙本质复合体层粘连蛋白的免疫组织化学研究  

IMMUNOHISTOCHEMICAL STAINING OF LAMININ IN DENTAL PULP-DENTIN COMPLEX OF HUMAN PREMOLAR

作　　者：薛敬玲[1] 朱世柱[1] 丁晓勇[1] 孟杨[1] 王纪[1] 尤建华[2] 

机构地区：[1]武汉大学医学院口腔解剖生理学教研室,武汉430071 [2]华中科技大学同济医学院同济医院,武汉430030

出　　处：《解剖学报》2002年第5期516-518,共3页Acta Anatomica Sinica

基　　金：湖北省教育厅自然科学类重点项目 (99A0 67)资助课题

摘　　要：目的　研究层粘连蛋白在牙髓牙本质复合体内的表达 ,探讨其分布与功能的关系。　方法　收集不同年龄新鲜健康人前磨牙 6 0颗 ,固定 ,脱钙 ,石蜡包埋 ,层粘连蛋白免疫组织化学染色。　结果　层粘连蛋白免疫反应性 ( IR)普遍存在于牙髓、前期牙本质和成熟牙本质小管与成牙本质细胞突起之间 ,以牙髓的神经与血管周围最为丰富 ,冠髓较根髓密集。　结论　层粘连蛋白 IR不仅见于牙髓与前期牙本质 ,而且也见于成熟牙本质 ,它对支持、固定牙髓牙本质复合体各结构的正常位置。Objective The expression of laminin was detected in dental pulp dentin complex of human premolar to find out the relationship between disribution and function of laminin. Methods Sixty human fresh and healthy human premolars were collected,fixed,decalcified,embedded in paraffin and cut into slices,then the slices were treated by immunohistochemical reaction of laminin. Results Laminin immunoreactivity was presented in dental pulp and predentin,and also in space between the wall of dentinal tubules and processes of odontoblast in mature dentin.It was most abundant around blood vessels and nerves in dental pulp,moreover,more abundant in crown pulp than in root pulp.Conclusions\ Laminin immunoreactivity usually appeared in dental pulp and predentin and also in mature dentin,suggesting that it may play an important role in maintaining normal form and position of pulp cells and other structures in dental pulp dentin complex.

关 键 词：牙髓牙本质复合体 层粘连蛋白 免疫组织化学 前磨牙 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R78[医药卫生—基础医学]