含与不含朱砂和雄黄的安宫牛黄丸对大鼠局灶性脑缺血的影响  

Effect of Angong Niuhuang Pill Containing or Not Containing Cinnaba and Realgar on Cerebral Focal Ischemia in Rats

作　　者：赵雍[1] 曹春雨[1] 王秀荣[1] 崔海峰[1] 王跃生[1] 王志民[1] 叶祖光 杜贵友[1] 

机构地区：[1]中国中医研究院中药研究所,北京100700 [2]国家药品监督局药品审评中心

出　　处：《中国中西医结合杂志》2002年第9期684-686,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家科技部"九五"攻关课题 (No .2 4 2 )

摘　　要：目的 :观察含与不含朱砂和雄黄的安宫牛黄丸对大脑中动脉栓塞模型大鼠脑梗塞面积、脑含水量及过氧化氢酶 (CAT)、脂质过氧化物 (LPO)、谷胱甘肽过氧化物酶 (GPX)、乳酸 (LD)含量的影响 ,探讨安宫牛黄丸中朱砂、雄黄对药效的影响。方法 :动物连续灌胃给药 3天后 ,采用大脑中动脉三氯化铁法造成大鼠脑局灶性缺血。分别用TTC染色法、烘干称重法、TBA法和按试剂盒说明书 ,测定大鼠脑梗塞面积、脑含水量和LPO、CAT、GPX、LD的含量。结果 :含与不含朱砂、雄黄的安宫牛黄丸各剂量组大脑中动脉栓塞大鼠脑梗塞面积明显减少 ;脑缺血侧含水量降低 ;CAT和GPX 含量增加 ,LPO和LD含量降低。结论 :含与不含朱砂、雄黄的安宫牛黄丸对大脑中动脉栓塞大鼠脑组织损伤均有一定的保护作用 ,两者之间未见显著差异 ,提示安宫牛黄丸中朱砂、雄黄对大鼠脑缺血梗塞的保护作用未有明显影响。Objective: To explore the influence of cinnaba and realgar in Angong Niuhuang Pill (ANP) on the effect of ANP by comparing the effects of ANP with or without cinnaba and realgar on the cerebral infarction area, water content, catalase (CAT), lipid peroxidase (LPO), glutathione peroxidase (GPx), lactic acid (LD) levels in brain of rat model of middle cerebral arterial occlusion (MCAO). Methods: Animal was made to form cerebral focal ischemia model by MCAO with FeCl 3, after medicated for 3 days, and the above mentioned parameters were determined separately by TTC stain, over dried and weighing, TBA method with test kit. Results: ANP, both with or without cinnaba and realgar, could reduce the area of cerebral infarction, brain water content, increase the level of CAT and GPx and lower the level of LPO and LD, suggesting that ANP could antagonize the free radical production in brain tissue. Conclusion: Both the ANP with or without cinnaba and realgar show protective effect on cerebral injury caused by MCAO, no significant difference is found between them, indicating that cinnaba and realgar have no influence on the protective effect of ANP on cerebral ischemic infarction in rats.

关 键 词：朱砂 雄黄 安宫牛黄丸 大鼠 局灶性脑缺血 影响 

分 类 号：R277.743.32[医药卫生—中医学]