用聚合酶链反应进行红色毛癣菌种内分型  

Strain Typing by PCR in Trichophyton rubrum

作　　者：李民[1] 王家俊[1] 章强强 李莉 兰和奎 任大明[2] 

机构地区：[1]上海复旦大学附属华山医院皮肤科真菌室 [2]复旦大学遗传所遗传工程国家重点实验室

出　　处：《中华皮肤科杂志》2002年第5期355-357,共3页Chinese Journal of Dermatology

摘　　要：目的建立一种快速且可重复的方法应用于红色毛癣菌的菌株区分。方法PCR扩增20株红色毛癣菌核糖体基因的非转录间隔区内的两个串联重复亚单位trs-1、trs-2,克隆两段PCR产物,用PGEM-T载体构建重组质粒载体进行目的基因测序。结果特异性扩增trs-1和trs-2区产生菌株特征的条带图。两个串联重复亚单位的基因测序结果说明了PCR指纹图差异的原因。结论这种PCR方法产生的特征性的指纹图提供了一种快速、稳定的分子分型方法,可应用于红色毛癣菌感染的流行病学和致病性的进一步研究。Objective To develop a rapid and reproducible m ethod in strain identification for T.rubrum.Methods Two novel tandem repeat subelements(TRSS),trs -1and trs -2,located in the T.rubrum rDNA non-transcribed spacer(NTS)were amplified from20strains of T.rubrum.The PCR products were cloned into seq uencing vector PGEM-T for sequencing.Results Specific amplification of trs -1and trs -2produced strain -characteris tic band patterns(PCR types),the sequence from trs -1and trs -2also supported this finding.The trs -1sequence variation was found in a strain of T.rubrum which can grow at 37℃.Conclusion The characteristic fingerprints generated by this PCR assay provide a rapid,stable molecular typing meth od to study the epidemiology and pathogenicity of T.rubrum infectio n.

关 键 词：聚合酶链反应 红色毛癣菌 种内菌株分型 流行病学 致病性 毛癣菌感染 基因分型 

分 类 号：R446.5[医药卫生—诊断学]