12株马内菲青霉随机扩增DNA多态性研究  

DNA Polymorphisium of Penicillium marneffei by Random Amplification PCR Assay

作　　者：吴易[1] 曹存巍[1] 李菊裳[1] 林有坤[1] 程鹂[1] 刘滔滔[2] 

机构地区：[1]广西医科大学第一附属医院皮肤科 [2]广西医科大学第一附属医院药剂科

出　　处：《中华皮肤科杂志》2002年第5期366-368,共3页Chinese Journal of Dermatology

摘　　要：目的了解马内菲青霉的基因学特征,为该菌的DNA分型提供依据。方法采用氯化苄提取法提取基因组DNA。用随机扩增多态性DNA(RAPD)技术对12株临床分离的马内菲青霉菌株进行DNA指纹图谱分析。结果①共选用40个随机引物进行扩增,筛选出4个具有稳定、清晰DNA扩增带型的引物。即引物P23:5'-AGTCAGCCAC-3',P103:5'-AGACGTCCAC-3',P105:5'-AGTCGTCCCC-3',P120:5'-GGGAGACATC-3'。②12株菌株DNA图谱主要带型相同,扩增片段呈多态性。相似率44.4%～97.3%。③12株菌可分5个不同的组群。结论RAPD技术可以用于马内菲青霉的基因分型。Objective To investigate the DNApolymorphism of Penicilliummarneffei(PM)and to provide an evidence for PMclassification.Methods Total DNAwas extracted with benzyl chloride.Randomamplification polymorphic DNA(RAPD)assay was used to study the finger-pr inting of 12strains Penicilliummarneffei.Results①Of forty randomprimers used,four of them:P 23 :5' -AGTCAGCCAC -3' ,P 103 :5' -AGACGTCCAC -3' ,P 105 :5' -AGTCGTCCCC -3' ,P 120 :5' -GGGAGACATC-3' gave good reactio n.②The major bands of DNApatterns of the12strains were of the same mobility.The amplified bands were polymorphic.The similarities were in the range of44.4%-97.3%.③12strains could be divided into 5gro ups.Conclusion RAPD may be applied in PMDNA classification.

关 键 词：马内菲青霉 随机扩增多态DNA技术 基因分型 马内菲青霉病 

分 类 号：R756[医药卫生—皮肤病学与性病学]