PVP/Pd/IrO_2/Nafion修饰微电极用于成纤维细胞中一氧化氮释放的研究  

Direct Monitoring of Nitric Oxide Release from Fibrocytes with PVP/Pd/IrO_2/Nafion Chemically Modified Microelectrode

作　　者：鲜跃仲[1] 徐继明[1] 陆嘉星[1] 刘梅川[1] 蔡琪[1] 金利通[1] 

机构地区：[1]华东师范大学化学系,上海200062

出　　处：《化学学报》2002年第8期1459-1464,共6页Acta Chimica Sinica

基　　金：国家自然科学基金 (No .2 9975 0 0 9);上海市自然科学基金 (No .0 0ZA14 0 11)资助项目

摘　　要：采用PVP Pd IrO2 Nafion修饰电极对成纤维细胞中NO的释放情况进行了研究 .结果表明 ,在正常状态下 ,采用NO前体L 精氨酸和乙酰胆碱对成纤维细胞进行刺激后没有NO的释放 ;当用脂多糖进行诱导后 ,则释放出高浓度的NO ,加入L 精氨酸和乙酰胆碱都促进了NO的合成 ,而L NNA的加入则逆转了L 精氨酸和乙酰胆碱的作用 .PVP/Pd/IrO2/Nafion microsensor was fabricated to measure nitric oxide production in fibrocytes directly. In the experiments, fibrocytes did not release nitric oxide after stimulated with L-arginine and acetylcholine under normal condition. After induced with hpopolysaccharide, NO was produced in high concentration and NO levels were increased after stimulated with L-arginine and acetylcholine. On the other hand, L-N-w-nitro-arginine, an NO synthase inhibitor, decreased the action of L-arginine and acetylcholine.

关 键 词：成纤维细胞 PVP/Pd/IrO2/Nafion修饰电极 一氧化氮 生物体 药物 生理 L-精氨酸 药理 病理 乙酰胆碱 

分 类 号：Q813[生物学—生物工程]