水稻细胞质雄性不育恢复系ZSP-1恢复基因的初步定位  

Mapping of Rice Fertility Restorer Genes for Cytoplasmic Male Sterility Detected in a Restorer Line ZSP-1

作　　者：杨存义[1] 陈乐天[1] 陈芳远[2] 刘耀光[1] 

机构地区：[1]华南农业大学生命科学院,广东广州510642 [2]广西大学水稻研究所,广西南宁530005

出　　处：《华南农业大学学报》2002年第4期30-33,共4页Journal of South China Agricultural University

基　　金：国家自然科学基金资助项目 (3972 5 0 16和 39980 0 2 7) ;国家科技部资助项目 (J99 A 0 0 2 ) ;广东省科技计划资助项目(B2 0 1)

摘　　要：调查了用细胞质雄性不育新恢复系ZSP 1配制的杂交组合珍汕 97A/ZSP 1和星A/ZSP 1的F1结实率及F2 个体花粉育性 ,发现恢复系ZSP 1的恢复性是由 2对独立遗传基因控制 .在此基础上 ,选用了与野败型雄性不育恢复基因Rf 3和Rf 4紧密连锁的RFLP标记对 2个F2 群体进行连锁分析 ,发现ZSP 1具有的 2个恢复基因与这些标记紧密连锁 ,初步把ZSP 1的恢复基因定位于Rf 3和Rf 4区域 .The pollen fertility of F 2 individuals of crosses Zhenshan 97A/ZSP 1 and Xing A/ZSP 1 was investigated. The segregation ratio of fertile to sterile plants was fit to 15∶1. The results show that fertility restoration of the cytoplasmic male sterility in these crosses was controlled by two independent restorer genes. The RFLP markers, that are linked closely to the known restorer genes Rf 3 and Rf 4 for WA CMS, were used to map the restorer genes found in the restorer line ZSP 1. The results of linkage analysis indicated that the restorer genes in ZSP 1 were located in the same regions of Rf 3 and Rf 4 .

关 键 词：水稻 细胞质 雄性不育 恢复系 ZSP-1 恢复基因 基因定位 RFLP分析 

分 类 号：S511[农业科学—作物学]