应用激光扫描共聚焦显微镜FRAP技术研究兔早期胚胎发育中细胞间隙连接介导通讯  

GAP JUNCTIONAL INTERCELLULAR COMMUNICATION (GJIC) BETWEEN RABBIT BLASTOMERES AS REVEALED BY LASER SCANNING CONFOCAL MICROSCOPY FRAP*

作　　者：徐营[1] 雷蕾[1] 刘忠华[1] 文端成[1] 廉莉[1] 朱子玉[1] 韩之明[1] 杨利国[2] 孙青原[1] 陈大元[1] 

机构地区：[1]中国科学院动物研究所计划生育生殖生物学国家重点实验室,北京100080 [2]南京农业大学动物科技学院,南京210095

出　　处：《细胞生物学杂志》2002年第5期310-312,共3页Chinese Journal of Cell Biology

基　　金：中国科学院知识创新重大项目(批准号:KSCX1-05-01);科技部攀登项目(批准号95-专-08)资助

摘　　要：本研究应用激光扫描共聚焦显微镜的光漂白恢复技术(FRAP)分析兔早期胚胎卵裂球之间通过间隙连接介导的细胞通讯(GJIC)。研究结果发现,用强激光分别将4-细胞期胚胎、异裂胚胎和8-细胞期胚胎的一个卵裂球荧光光漂白后,经过15分钟的荧光恢复,4-细胞期胚胎的光漂白恢复率为17.8％,异裂胚胎的光漂白恢复率为23.7％,二者之间没有明显的差异;8-细胞期胚胎的光漂白恢复率为78.2％,与前二者之间存在明显的差异。推测兔早期胚胎卵裂球细胞间隙连接建立的时间在8-细胞阶段,胚胎卵裂球间隙连接通讯可能是兔胚胎正常发育的重要条件。In order to analyze the gap junctional intercellular communication(GJIC) between rabbit blastomeres, we observed the fluorescence redistribution after photobleaching(FRAP)by laser scanning confocal microscopy. The results showed that the recovery rate was 17.8% in 4-cell stage embryos,23. 7% in the abnormal embryos,after 15 min and that there was no significant difference between them. The recovery rate of 8-cell embryos was 78.2% .significantly higher than that of 4-cell or abnormal embryos. The result indicated that the gap junction between blastomeres may be established at 8-cell stage,and it may be important to the development of rabbit embryos.

关 键 词：激光扫描共聚焦显微镜 FRAP技术 兔 早期胚胎发育 细胞间隙连接介导 细胞间通讯 

分 类 号：Q6-3[生物学—生物物理学]