人外周血中SLA-P1抗原特异性T细胞前体频率及其细胞因子分泌格局分析  

Analysis of the Precursor Frequency and Cytokine Profiles of SLA Class I P1-Specific T Lymphocytes in Human Peripheral Blood

作　　者：唐军[1] 周芸[1] 陶箭[1] 张勇[1] 王胜旺[1] 周光炎[1] 

机构地区：[1]上海第二医科大学上海市免疫学研究所,上海200025

出　　处：《上海免疫学杂志》2002年第5期301-303,共3页Shanghai Journal of Immunology

基　　金：国家自然科学基金重大资助项目(No 399934 30 2 )

摘　　要：用基因工程表达并纯化的版纳猪SLAI类P1为抗原 ,经两轮刺激PBMC以3H TdR法检测 1 0名健康人外周血中的特异性T细胞前体频率在 6 67× 1 0 7～ 1 0 8× 1 0 6 之间。用ELISA与RT PCR法进一步分析了抗原特异性细胞的细胞因子分泌特点 ,发现所有抗原阳性孔都呈现IFN γ (+) /IL 4( )的典型Th1型格局。以上结果部分明确了外周血SLA P1特异性T细胞的特征 ,为揭示SLA P1特异性T细胞在猪Using recombinant Banna Pig SLA class I P1 molecules as antigen,with two rounds of stimulation in vitro,the frequencies of the precursor T lymphocytes in PBMC from 10 healthy individuals were identified,ranging from 6 67×10 -7 to 1 08×10 -6 In a meanwhile,the cytokine profiles of SLA P1 specific T lymphocytes were assayed using ELISA and RT PCR All antigen positive wells showed the typical Th1 type cytokine profile,i e IFN γ (+)/IL 4 ( ) The results partly identified in vitro the characteristics of SLA P1 specific T lymphocytes in healthy PBMC,indicating that SLA P1 specific T lymphocytes were possibly responsible for xeno acute cellular rejection

关 键 词：SLA T细胞前体频率 细胞因子格局 基因工程抗原 ELISA法 RT-PCR法 组织细胞移植 细胞性排斥 

分 类 号：R392.12[医药卫生—免疫学] R392.4[医药卫生—基础医学]