小檗碱在TLC原位的FT-SERS研究  

In Situ Thin Layer Chromatography-fourier Transform-surface-enhanced Raman Spectrum Study on Ingredients of Berberine

作　　者：汪瑗[1] 国占生[2] 王英锋[1] 王松颖[3] 任桂芬[1] 张湘兰[1] 韩秀兰[1] 

机构地区：[1]首都师范大学化学系,北京100037 [2]衡水师范专科学校化学系,河北衡水053000 [3]北京理工大学物理系,北京100081

出　　处：《光谱学与光谱分析》2002年第5期745-748,共4页Spectroscopy and Spectral Analysis

基　　金：北京中关村地区联合测试中心测试基金;首都师范大学重点科研项目资助

摘　　要：应用表面增强技术将薄层色谱与近红外傅里叶变换拉曼光谱联用 ,获得了中成药三黄片中主要有效成分小檗碱在薄层原位的傅里叶变换表面增强拉曼光谱 (FT SERS)。研究表明 ,在薄层原位约 2 5 μm样品的FT SERS与纯固体样品的FT Raman光谱的主要特征峰波数基本一致 ,相对强度有一定变化 ,出现特别增强的拉曼峰是波数为 72 7nm的芳香环上CH键面内变形振动峰。小檗碱分子以异喹啉环共轭体系的π电子与表面增强活性物质银晶体微粒相互作用 ,呈平面吸附模式。Surface Enhanced Raman Scattering (SERS) combined with Thin Layer Chromatography (TLC) has been used for studying characteristic spectrum of molecules in situ in micrograms samples. There are very few report for applying the SERS-TCL method in the study of the effective ingredients of Chinese traditional herbs. Coptis Chinensis France is an often-used clinic Chinese traditional medicine. Its main effective components include berberine and so on, which have antibiotic very wide and also have curative effect on improving the functions of heart vascular cycles. Therefore the concentrations of berberine are very important for the quality control of the medicine. In this work, the ethanol extract of Coptis Chinensis France was first separated by TLC, the SERS was then measure directly after dropping silver gel on the separated spots. The method can be used for the finger print analysis of the berberine. 3 muL of alcohol extract of Coptis Chinensis Franch with total. alkaloids concentration of 1.0 mg . mL was placed on silicon GF(254)TLC plate. The sample was separated by developing solvent of n bulanol-Acitic acid-H2O (7:2: 1 V/V). The positions of berberine in the sample were confirmed by the standard alkaloid solutions. The Rf values for berberine are 0.29. The silver gel was used as surface enhanced substrate and placed on the separated berberine spots. FT-SERS was measured directly by a Nicolet FT-Raman 910 spectrometer. Berberine belong to isoquinoline alkaloids. His structure can be found in reference. The date of spectrum of berberine can be seen that the band at 1396 cm(-1) due to Ar-OCH3 deformation vibrations was greatly enhanced, indicating that the molecule was absorbed on silver gel strongly through lone-pair electron in An OCH3. The ring stretching mode occurring around 1 548 cm(-1) represents isoquinoline ring in the molecule. The band at 727 cm(-1) due to CH (ring) deformation vibrations was also enhanced.

关 键 词：TLC FT-SERS 薄层色谱 傅里叶变换表面增强拉曼光谱 小檗碱 中成药 三黄片 分析 

分 类 号：R284.1[医药卫生—中药学]