一种新型蛋白聚糖类生物絮凝剂的分离纯化及组成分析  

PURIFICATION AND IDENTIFICATION OF NOVEL BIOFLOCCULANT

作　　者：何宁[1] 李寅[1] 陈坚[1] 伦世仪[1] 

机构地区：[1]无锡轻工大学生物工程学院环境生物技术研究室,江苏无锡214036

出　　处：《化工学报》2002年第10期1022-1027,共6页CIESC Journal

基　　金：教育部跨世纪人才培养计划基金资助项目

摘　　要：从土壤中筛选获得了一株絮凝剂产生菌 ,经初步鉴定为诺卡氏菌 (Nocardiasp .) .考察了该菌株的基本生长代谢特性 ,其合成的絮凝剂约 80 %以上分泌至胞外培养基中 .利用有机溶剂沉淀结合柱层析法从Nocardiasp .CCTCCM 2 0 10 0 5的发酵液中提取到一种新型生物絮凝剂REA - 11.分析表明 ,REA -This paper mainly discussed a flocculant-producing bacteria isolated from soil and the flocculating substance secreted into the medium. Preliminary studies identified the bacteria CCTCC M201005 as Nocardia species. Its growth and metabolism were studied. More than 80% of the flocculating activity was detected in the culture broth. A new bioflocculant-RFA-11 was purified by repeated ethanol precipitation, ion exchange and gel chromatography from the culture broth. Its molecular mass was estimated to be over 105 g&middot mol-1 by the gel filtration method. The results of investigation on the purified REA-11 and its acid hydrolysate by colcrimetric analysis, paper chromatography, HPLC and GC-MS suggested that REA-11 was a novel acidic polysaccharide mainly composed of galactocuronic acid with little acetic acid but no hexosamine detected. Protein was not a necessary part for the flocculating activity of REA-11, but mainly assisting in connecting the monosaccharides in the macromolecule or maintaining the dimensional conformation of the active biopolymer.

关 键 词：蛋白聚糖类生物絮凝剂 分离 纯化 组成分析 诺卡氏菌 产菌菌株 水处理 

分 类 号：TU991.22[建筑科学—市政工程] X703.1[环境科学与工程—环境工程]