铽-敏化荧光法测定妥舒沙星  

Terbium sensitized fluorescence determination of tosufloxacin

作　　者：赵慧春[1] 冯瑞琴[1] 王晓斌[1] 易琳[1] 

机构地区：[1]北京师范大学化学系,北京100875

出　　处：《分析试验室》2002年第5期2-4,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金资助项目 (2 99710 0 5 );北京市自然科学基金项目 (2 0 2 2 0 07)

摘　　要：依据Tb3+和妥舒沙星 (TSLX)形成配合物并在分子内发生能量转移 ,产生Tb3+的特征荧光 ,建立了一种分析妥舒沙星含量的的新方法。方法的线性范围为 7.0× 1 0 - 8～ 5 .0× 1 0 - 6 mol·L- 1 ,检出限为 8.5× 1 0 - 9mol·L- 1 。测定了胶囊中妥舒沙星的含量和回收率 ,回收率在 99.8%～ 1 0 4 %之间。并对Tb3+ 妥舒沙星体系敏化荧光的机理进行了探讨。The determination of tosufloxacin(TSLX) was studied by sensitized fluorescence method. Intramolecular energy transfer occured in Tb 3+-TSLX complex and the fluorescence of Tb 3+ was sensitized by TSLX. The fluorescence intensity of Tb 3+-TSLX system was proportional to the amount of TSLX. Under the optimum experimental conditions, the linear range and detection limit for TSLX were 7.0×10 -8～5.0×10 -6 mol·L -1 and 8.5×10 -9 mol·L -1, respectively. This method was successfully applied to the determination of TSLX in capsule without any pretreatment.

关 键 词：妥舒沙星 敏化荧光法 铽 抗菌药物 分析 

分 类 号：R978.1[医药卫生—药品] R917[医药卫生—药学]