聚中性红作电子转移介体的过氧化氢生物传感器  

A Hydrogen Peroxide Sensor Modified by Poly(neutral-red)as the Mediator

作　　者：张国荣[1] 刘海燕[1] 王艳玲[1] 

机构地区：[1]山东师范大学化学系,济南250014

出　　处：《分析化学》2002年第10期1237-1239,共3页Chinese Journal of Analytical Chemistry

摘　　要：提出一种新的第二代电流型生物传感器。将易溶于水的电子转移介体中性红经电聚合在石墨电极表面形成一层致密、稳定的膜 ,有效的减少了其流失。并在辣根过氧化酶的表面覆盖一层明胶 ,再用戊二醛交联制备了H2 O2 传感器 ,使固定化酶保持了较高活性。该生物传感器具有较宽的线性范围 :2 .0× 10 - 5～ 2 .5×10 - 2 mol L ;检出限为 1.8× 10 - 5mol L ,达到 95 %稳态电流所用时间 <2 0s。A paraffin soaked graphite electrode was fabricated by poly (neutral red) film, covering with a layer of gelatin, and finally cross-linking with glutaraldehyde. The method greatly shorten the running-off of water-soluble mediator, nutral red, and improved the stability. The fabricated H, 0, sensor presents a linear range from 0.02 mmol/L to 25 mmol/L H2O2; The response time and the detection limit were 30s, 0.018 mmol/L respectively. The sensor retains more than 90% of initial response after storage (4degreesC) for four months.

关 键 词：电子转移介体 过氧化氢 聚中性红 明胶 辣根过氧化酶 生物传感器 浸腊石墨电极 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]