Involvement of gene expressions in apoptosis of vascularendothelial cells induced by rattlesnake venom  

作　　者：MIAO JUN YING SATOHIKO ARAKI YI RENHAN HIROSHI HAYASHI(Institute of DeveloPmental Biology, School of Life Sci-ence, Shandong University, Jinan 250100, China)(Sugashima Marine BiolOgical Labomtory, School of Sci-ence, Nagoya University, To6a, Me, 517 Japa 

出　　处：《Cell Research》1999年第3期237-242,共6页细胞研究（英文版）

摘　　要：Formation of apoptotic bodies is a typical character ofaPoptotic cell death, but how the processes are controlledis not known. In this study, we compared two apoptosisinducing systems in vascular endothelial cells (VEC). Wefound that the formation of aPoptotic bodies during apop-tosis induced by rattlesnake venom, which is an unique andspecific aPoptosis inducer to vascular endotheliaI cells, wasmuch faster than that induced by deprivation of survivalfactors (aFGF and serum). When we blocked the synthesisof mRNAs in cells treated with rattlesnake venom by DRB(5, 6- dichloro- 1 -β- D- rib ofur anosylb enzimidazole ), an in-hibitor of transcription, the formation of aPoptotic bodieswas dramatically inhibited. We examined the expressionof Psa gene and found that its expression was much higherin apoptosis induced by rattlesnake venom than that inaPoptosis induced by deprivation of aFGF and serum. Ourresults suggest that gene expression is important and P53gene may play a major role in inducing the formation ofapoptotic bodies in VEC.

关 键 词：APOPTOSIS ratt1esnake venom gene expressions P^53 gene endothelia1 cells 

分 类 号：Q255[生物学—细胞生物学] Q26