HPLC测定咳必安胶囊及其白云花根中欧前胡素的含量  

Determination of imperatorin in Kebian capsules and Heracleum rapula by HPLC

作　　者：郑纯[1] 黄以钟[1] 徐振国[2] 

机构地区：[1]福建省药品检验所,福建福州350001 [2]福建中医学院药学系,2001届实习生福建福州350003

出　　处：《中国药学杂志》2002年第10期778-781,共4页Chinese Pharmaceutical Journal

摘　　要：目的　采用RP HPLC ,流动相梯度洗脱对咳必安胶囊及白云花根药材进行HPLC指纹图谱初步研究 ,制定白云花根药材香豆素成分的指纹图谱 ,探讨制剂及其药材香豆素成分指纹图谱的一致性。建立HPLC测定咳必安胶囊和白云花根药材中欧前胡素含量的方法。方法　采用 10 %氨水前处理 ,流动相 :甲醇 水 (6 5∶35 ) ,色谱柱 :Nova PakC18柱 (2 5 0mm× 4 .6mm ,4 μm) ,检测波长 :2 4 9nm。 结果　线性范围 :0 .0 77～ 0 .77μg(r =0 .9999) ;精密度RSD为 0 .17% ,日内、日间重现性RSD均小于 3% ;最低检测限为 0 .15 μg·mL-1。平均回收率为 98.5 2 % ,RSD为 1.82 %。结论　本法快速、简便 ,精密度、重现性好 ,灵敏度高 。OBJECTIVE: To establish a HPLC-fingerprint identification method of Heracleum rapula Franch, and investigate the relativity of HPLC-fingerprint for the herb and Kebian capsules. To establish a RP-HPLC method for the determination of imperatorin in Kebian capsules and Heracleum rapula Franch. METHODS: The HPLC-fingerprint of coumarin constituents of Herb and its preparation were analysed with gradient mobile phase of methanol-water. The samples were purified by 10 % ammonia. The determination of imperatorin was carried out on C18 Nova-Pak column with the mobile phase of methanol-water (65:35), and the detection wavelength was at 249 nm. RESULTS: A good linearity was obtained from 77.02 to 770.2 μg with a correlation coefficient of 0.999 9. The detection limit was 0.14 μg&middotmL-1. The RSD of inter-day and intra-day was less than 3% (n = 6). The average recovery of the assay was 98.5% with RSD 1.82% (n = 6). CONCLUSION: The method appeared to be simple, accurate, reproducible, and can be used for quality control of Heracleum rapula Franch, and its preparation.

关 键 词：咳必安胶囊 白云花根 含量测定 高效液相色谱法 欧前胡素 

分 类 号：R927.2[医药卫生—药学] R284.1