应用PCR-SSCP技术检测PGM_1基因型  

PGM_1 genotyping by PCR-SSCP

作　　者：宋海燕[1] 杨庆恩[1] 余纯应[1] 

机构地区：[1]华中科技大学同济医学院法医学系,湖北武汉430030

出　　处：《法医学杂志》2002年第3期152-154,共3页Journal of Forensic Medicine

摘　　要：目的应用PCR-SSCP技术分型PGM1基因型。方法提取156份武汉地区汉族无关个体的血样DNA,分别扩增PGM1基因外显子4和外显子8的多态性靶DNA,用SSCP分析PCR产物,判断基因型。结果两种PCR产物均检出了两个等位基因、三种基因型,DP值分别为0.5620、0.4405。综合外显子4和8的PCR-SSCP结果,分出8种PGM1基因型,DP值为0.7318。应用本法对保存10年的陈旧血痕和精斑PGM1分型成功。结论用PCR-SSCP分型PGM1基因型在法医物证检验中具有实用价值。Objective PGM 1 genotyping by PCR-SSCP analysis.Methods Amplified genome DNA from156unrelated Han individuals living in Wuhan,PCR products for exon4and exon8of PGM 1 were then analyzed by SSCP to detect the genotypes.Results2alleles and3genotypes were detected in exon4and8respectively.The discrimination power was0.7318.PCR-SSCP analysis was suitable for determination of PGM 1 genotypes from old blood and semen stains.Conlusion PGM 1 system typed by PCR-SSCP is useful for forensic identification.

关 键 词：磷酸葡萄糖变位酶1 基因型 PCR-SSCP 法医物证检验学 

分 类 号：D919.2[医药卫生—法医学]