胶原凝胶真皮构建及其性质测定  

Construction of a Collagen Gel Dermis with Improved Biological Property

作　　者：刘玲蓉[1] 张立海[1] 马东瑞[1] 张其清[1] 

机构地区：[1]中国医学科学院中国协和医科大学生物医学工程研究所生物材料研究室,天津300192

出　　处：《中国医学科学院学报》2002年第4期373-376,I003,共5页Acta Academiae Medicinae Sinicae

基　　金：国家重点基础研究发展规划项目(G199054309);天津市自然科学重点基金(003802511)资助~~

摘　　要：目的为了改进组织工程化人工皮肤的生物学性能。方法改进Hansburgh和Middelkoop制备人工真皮的方法,应用I型胶原酶和中性蛋白酶Dispase分离得到新生儿真皮成纤维细胞,并将其种植于胶原溶胀液,成功构建凝胶真皮,并检测了其力学及生物学性能。结果胎儿真皮成纤维细胞在凝胶真皮中增殖速度快、活性高、分泌细胞外基质丰富。结论凝胶真皮的生物学性能优于传统胶原海绵人工真皮。Objective To improve the biological property of artificial skin.Methods We have ameliorated Hansburgh and Middelkoop's method of manufacturing artificial dermis.The type I collagenase and Dispase were used to isolated neonate prepuce'dermis fibroblast.The gel dermis was constructed by compounding the fibroblast and collagen swelling solution.The property of the collagen gel dermis was measured.Results The neonate prepuce's dermis fibroblast had property of high proliferation,high activation of the dermis,and it could secrete abundant extracellular matrix(ECM).Conclusion The collagen gel dermis is an useful dermis substitute.

关 键 词：胶原凝胶 真皮成纤维细胞 皮肤组织工程 生物力学 

分 类 号：R318.19[医药卫生—生物医学工程]