应用PCR-SSP技术对云南拉祜族人HLA-DRB1进行基因分型  

Genotyping of HLA-DRB1by PCR-SSP in Yunnan Lahu Ethnic Groups

作　　者：洪坤学 何勤 单西云 俞建昆[4] 林克勤[4] 史磊[4] 褚嘉佑[4] 

机构地区：[1]National Center for AIDS Prevention and Control,beijing100050 [2]The Second Affiliated Hospital,Kunming MedicalColledge,kunming650118 [3]The Prefecture Hospital of Xishuangbanna,jinghong666102 [4]中国医学科学院中国协和医科大学医学生物学研究所遗传室,昆明650118

出　　处：《中国医学科学院学报》2002年第4期389-392,共4页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金重大项目(39993420);美国中华医学基金(CMB98-675)资助~~

摘　　要：目的了解云南拉祜族健康人HLA-DRB1基因的遗传多态性。方法应用PCR-SSP技术对110名无亲缘关系的云南拉祜族健康人进行HLA-DRB1基因分型。结果鉴定了拉祜族群体HLA-DRB1位点的16种等位基因,包括DRB1位点目前已知的全部血清学特异性,经检验DRB1位点的基因型分布符合Hardy-Weinberg平衡(P>0.75)。结论提供了一套比较完整准确的云南拉祜族群体DRB1等位基因的基因频率,对群体遗传和疾病关联研究具有参考意义。Objective To investigate polymorphism of HLA-DRB1in Chinese Lahu population in Yunnan.Methods Polymerase chain reaction-sequence specific primers(PCR-SSP)were used to determine HLA-DRB1genotypes of110unrelated healthy Lahu minority people of Yunnan Province.Results Sixteen alleles of DRB1were detected in this study.The results of test showed that the genotype distributions observed were corresponded with the Hardy-Weinberg equilibrium.Conclusion This study has obtained a more comprehensive and accurate data set of the normal allele frequencies of HLA-DRB1in Chinese Lahu population in Yunnan Province,which may be of significance in the studies on population genetics and disease association.

关 键 词：PCR-SSP技术 云南 拉祜族人 HLA-DRB1 基因分型 群体遗传学 

分 类 号：R392.2[医药卫生—免疫学]