紫外分光光度法测量皮肤及血液中盐酸罂粟碱含量  

Determination of Papaverine Hydrochloride in Skin and Blood and the Drug Contents in Pig Skin

作　　者：唐勇[1] 栾杰[1] 王黔[1] 乌丽[1] 曹蕊[1] 刘淑红[1] 

机构地区：[1]中国医学科学院中国协和医科大学整形外科医院南二病房,北京100041

出　　处：《中国医学科学院学报》2002年第4期413-417,共5页Acta Academiae Medicinae Sinicae

摘　　要：目的探讨以紫外分光光度法测量血液及皮肤组织中盐酸罂粟碱含量及外用罂粟碱后局部皮肤组织中的药物含量。方法将pH=10的磷酸缓冲液加入含盐酸罂粟碱的血液或研碎的皮肤组织中,以氯仿-己烷(2∶3)混合液萃取其中的盐酸罂粟碱,经振荡离心后,取部分有机相经60℃水浴蒸干,以1mol/L的盐酸溶解蒸干物,经孔径0.2μm的滤膜过滤,以紫外分光光度法测量其中盐酸罂粟碱的浓度。将含2%的盐酸罂粟碱霜外用于实验猪上,定时切取标本以紫外分光光度法测量其皮肤组织中的药物含量。结果萃取物的吸收光谱呈典型的盐酸罂粟碱吸收光谱,无杂峰出现。血液中盐酸罂粟碱萃取率达92%以上,皮肤组织萃取率达87%以上。将2%盐酸罂粟碱霜外用于实验猪皮肤8h后,皮肤组织中盐酸罂粟碱含量达90μg/g以上,远高于罂粟碱有效药物浓度。结论紫外分光光度法测量血液及皮肤组织中盐酸罂粟碱含量是一种简便、经济、可靠的方法,有较好的重复性和较高的灵敏度。外用罂粟碱霜可渗透到皮肤中并达到有效药物浓度。Objective To explore the application of ultraviolet spectrophotometer in determining papaverine hydrochloride content in blood and skin and to determine the intradermal content after local external application of papaverine hydrochloride.Methods Phosphate buffer solution(pH=10,ionic strength=0.4)was added into the blood or ground skin tissue containing papaverine hydrochloride,and papaverine hydrochloride was extracted with chloroform-hexane mixture(ratio:2∶3).After oscillation and centrifugation,partial organic phase was retrieved and dried in60℃water bath.The dried product was dissolved in1mol/L HCl and then underwent filtration through0.2μm membrane.The ultraviolet apectrophotometry was applied to determine the content of papaverine hydrochloride in the filtrate.2%papaverine hydrochloride cream was applied on pig' s skin and skin specimen was retrieved at given time to determine the papaverine hydrochloride content in skin tissue with ultraviolet spectrop-hotometry.Results The absorbance spectrum of the extract was typical of that of papaverine hydrochloride,with no miscellaneous peak.The extracive rate of papaverine hydrochloride in blood of was more than92%and that in skin tissue more than87%.Eight hours after external application of2%papaverine hydrochloride cream on pig skin,the content of papaverine hydrochloride in skin tissue was more than90μg/g,significantly higher than the effective concentration of papaverine hydrochloride.Conclusions Ultraviolet spectrophotometry is a simple,economical and reliable method for the determination of papaverine hydrochloride content in blood and skin tissue,with good reprod-ucibility and sensibility.External application of papaverine hydrocholine cream can provide efficient permeation and drug concentration in the skin.

关 键 词：紫外分光光度法 盐酸罂粟碱 血液 皮肤 药物含量 血管松弛剂 

分 类 号：R969[医药卫生—药理学] R972[医药卫生—药学]