肺炎克雷伯菌下呼吸道感染的耐药性及基因分型研究  

Drug resistance and genotyping of Klebsiella pneumoniae in lower respiratory tract infection

作　　者：施金玲[1] 李艳[1] 李从荣[1] 蔡璇[1] 李红霞[1] 彭少华[1] 

机构地区：[1]武汉大学人民医院检验科,武汉430060

出　　处：《中华结核和呼吸杂志》2002年第10期607-609,共3页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的　了解肺炎克雷伯菌下呼吸道感染株的耐药现状 ,监测耐药株的流行趋势。方法　采用微量稀释法对 86株引起下呼吸道感染的肺炎克雷伯菌进行抗生素药敏试验和超广谱 β 内酰胺酶 (ESBLs)检测 ,并对产ESBLs菌株以随机扩增多态性DNA(RAPD)法进行基因分型。结果　产ESBLs肺炎克雷伯菌占 2 0 9% ,其中 83 3 %来源于呼吸科重症监护病房 (RICU)。ESBLs阳性菌对 1 0种抗生素的耐药性远远高于ESBLs阴性菌 ,并同时高耐氨基糖苷类、喹诺酮类抗生素。 1 8株产ESBLs肺炎克雷伯菌分为 1 2型。其中 3株属于A型 ,5株属于B型 ,其它为不同类型。结论　RICU病房有产ESBLs肺炎克雷伯菌同一克隆株集中分布 。Objective To understand the current drug resistance of Klebsiella pneumoniae in lower respiratory tract infection and the prevalence trend of extended spectrum beta lactamase (ESBLs) producing Klebsiella pneumoniae Methods Drug resistance in 86 strains of Klebsiella pneumoniae and ESBLs producing strains were genotyped by randomly amplified DNA polymorphisms (RAPD) Results ESBLs producing strains accounted for 20 9% of the 86 strains, and 83 3% of the ESBLs producing strains were from respiratory ICU The drug resistance rate of ESBLs positive bacteria was much higher than that of ESBLs negative bacteria ESBLs positive bacteria were resistant to both the quinolones and a minoglylosides Eighteen ESBLs producing strains were divided into 12 types 3 strains had the same profile (genotype A), and 5 strains had an identical profile (genotype B), but other strains had different profiles Conclusions Chromosomal DNA analysis proved that certain types of ESBLs producing Klebsiella pneumoniae were epidemic in respiratory ICU.RAPD was an economic, simple but reliable method in epidemic study

关 键 词：流行病学 肺炎克雷伯菌 下呼吸道感染 耐药性 基因分型 

分 类 号：R181.3[医药卫生—流行病学]