补气补血方剂对创伤应激鼠的调理作用  

Effects of Chinese Medlicinal Herbs of Invigorating Function in Traumatized Mice

作　　者：郝江[1] 文家福[1] 潘兴华[1] 宁亚功[1] 乌胜平[1] 

机构地区：[1]成都军区昆明总医院,昆明650032

出　　处：《中国中西医结合外科杂志》2002年第5期369-371,共3页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

摘　　要：目的 　观察补气补血方对创伤鼠应激反应的影响 ,探讨作用机理。 方法 　随机将小鼠分为正常、对照、治疗 1组(当归 )、治疗 2组 (大黄 )和治疗 3组 (当归 +大黄 )。治疗及对照组落体撞击法造成双侧股骨闭合性骨折 ,并 2次 /d胃灌入中药或生理盐水 ,伤后第 1、2、5d取血放免法测定血β 内啡肽、皮质醇含量。以上各组均于第 5d测脾淋巴细胞对PHA的增殖反应。 结果 　治疗 1、3组脾淋巴细胞对PHA的增殖反应较对照组明显改善 (P <0 .0 1) ,治疗 1组血 β -EP、Cor升高得到控制 ,(P <0 .0 5 ) ;治疗 2组PHA无明显改善 ,血Cor抑制 ,β EP逐步升高 ;治疗 3组以上应激素变化较 2组缓和。 结论　 以补气补血为主的扶正方剂 ,可通过双向调节作用 ,改善机体对创伤应激的反应。Objective:To study the changes of stress hormone and immunity function in leg-fractured mice,and evaluate the effects of TCM formulae of invigorating vitality and enriching blood Methods:Kunming mice were randomly divided into normal(Ⅰ),traumatized control(Ⅱ),treated first(Dang Gui,Ⅲ),treated second(Da Huang,Ⅳ),and treated third(Dang Gui plus Da Huang Ⅴ)groups.Mice of Ⅱ-Ⅴ groups were made closed fracture on two femurs by method of dropping sunstance strike.Then th mice ecept in Ⅰ group were treated with herbal medidine or physiological saline given through gastric tube respectively.Seven mice in each group were killed on day 1,2 and 5 after trauma,and plasma β-endorphin(β-EP)and cortisone concentrations were measured by radioimmuno-assay(RIA).The proliferative activity of spleen T cells was measured by MTT assay on the fifth day.Results:The proliferative activity of T cells in Ⅲ and Ⅴ group was significantly higher than that in traumatized control group.The level of β EP and cortisone in Ⅲ and Ⅴ group was controlled or improved.But in group Ⅳ cortisone was restrained and β EP increased.The activity of T cells in group Ⅳ was not improved.Conclusion:The TCM formulae of invigorating vitality and enriching blood through the way of bi-directional regulation,treating the excess-syndrome by prugation and simultaneously treating the deficiency-syndrome by invigoration to modulate and improve the stress response of mice for trauma.

关 键 词：补气补血方剂 创伤 皮质醇 应激反应 Β-内啡肽 作用机理 大鼠 放射免疫法 

分 类 号：R289.5[医药卫生—方剂学] R269.4[医药卫生—中药学]