口服精氨酸对烫伤大鼠肠缺血—再灌注损害的保护作用  

Protective effects of oral arginine on gut ischemia-reperfusion injury in rats with scald

作　　者：陈斌[1] 付晋凤[1] 陈宗华[1] 许琰[2] 汪虹[1] 李翠萍[1] 

机构地区：[1]昆明医学院第二附属医院烧伤科云南省烧伤研究中心,650101 [2]昆明医学院免疫学与微生物教研室

出　　处：《中国急救医学》2002年第10期567-568,共2页Chinese Journal of Critical Care Medicine

基　　金：云南省卫生厅 1999年科研基金资助项目 (No 99Q0 3 5 )

摘　　要：目的　探讨口服精氨酸对烫伤大鼠肠缺血—再灌注损害的保护作用。方法　 6 6只SD大鼠随机分为正常对照组 (N组 ,6只 )、灌喂精氨酸组 (A组 ,30只 )和普通喂养组 (E组 ,30只 )。A、E组复制 30 %体表总面积 (TBSA)Ⅲ度烫伤模型。分别观察伤后 6、12、2 4、4 8、72h肠组织超氧化物歧化酶 (SOD)、丙二醛 (MDA)、一氧化氮 (NO)含量的变化以及肠外组织器官的细菌移位率。结果　与N组比较 ,A、E组烫伤后肠组织SOD活力明显下降 (P <0 0 5 )、MDA含量显著上升 (P <0 0 5 ) ,但A组的这种变化低于E组 ,尤其是在伤后 12、2 4h(P <0 0 5 )。A组伤后 2 4h内肠组织NO含量明显高于E组 (P <0 0 1)。伤后2 4、4 8hA组细菌移位率明显低于E组 (P <0 0 5 )。结论　口服精氨酸可以减轻烧伤后肠缺血—再灌注所引起的肠黏膜屏障损害 ,其机制可能与口服精氨酸导致肠黏膜局部NO含量增加有关。Objective To explore the protective effects of oral arginine on gut ischemia-reperfusion injury in rats with scald.Methods Sixty-six SD rats were randomly divided into normal control(N,n=6),oral L-arginine(A,n=30) and normal enteral feeding(E,n=30).The rats of group A and E were inflicted with 30% total body surface area full thickness scald.The content of superoxide dismutase (SOD),malondialdehyde(MDA),nitric oxide (NO) in intestinal mucosa and bacterial translocation rates of extraintestinal organs.Results Compared with N group,the content of SOD in intestinal mucosa was decreased significantly (P<0 05) and the content of MDA in intestinal mucosa was increased significantly(P<0 05) after scald both A and E groups.Compared with E group,the changes of SOD and MDA were lower than E group and it was significant on 12 h and 24 h after scald.The content of NO in intestinal mucosa was obviously higher than that in E group on 24 h after scald (P<0 01).Bacterial translocation rates of A group were decreased significantly than E group on 24 h and 48 h after scald.Conclusions Oral arginine may relieve the damage of gut barrier after the postburn gut ischemia-reperfusion.The mechanism of this protective effect may be correlated with increased the content of NO in intestinal mucosa by oral L-arginine.

关 键 词：口服 烫伤 肠道缺血-再灌注损害 保护作用 一氧化氮 肠黏膜 动物实验 左旋精氨酸 

分 类 号：R965[医药卫生—药理学]