^(60)Coγ射线对体外培养成骨细胞作用的实验研究  

Effect of ^(60)Coγ-ray irradiation on in vitro cultured osteoblasts

作　　者：李建福[1] 程天民[1] 冉新泽[1] 林远[1] 

机构地区：[1]第三军医大学预防医学系防原医学教研室,全军复合伤研究所,重庆400038

出　　处：《第三军医大学学报》2002年第9期1005-1008,共4页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划资助项目 ("973"项目 ) (G19990 54 2 0 5)

摘　　要：目的　研究放射性骨损伤的发病机制 ,探讨骨折合并射线损伤愈合能力下降的原因。方法　将体外培养的成骨细胞用60 Coγ射线一次性照射 3、6、9、12Gy ,以建立体外培养成骨细胞受射线作用的实验模型 ;同时 ,观察成骨细胞的碱性磷酸酶 (ALP)活性改变 ,骨形态发生蛋白 (BMP)和转化生长因子 β1(TGF β1)的胞质内表达量 ,以及细胞内钙离子的含量。结果　成骨细胞受射线照射后 ,其ALP活性明显降低 ,且与照射剂量呈明显量效关系 ,3Gy组抑制率为 3 1 5 9% ,而 12Gy组则为 84 95 % ;BMP、TGF β1的表达量下降 ,但与照射剂量无明显量效关系 ;胞质内钙含量亦降低 ,但受照各组间的抑制率无明显统计学差异。结论　射线对成骨细胞具有直接损害作用 ,放射性骨损伤的重要方面就是射线对成骨细胞的主要功能酶ALP活性的损害 ,抑制BMP、TGF β1等生长因子的合成 ,且干扰细胞内第二信使钙离子 。Objective To explore the pathogenesis of irradiation induced bone injury and the cause of the reduction of bone regenerative capacity in fracture of bone combined with irradiation injury. Methods The cultured osteoblasts were irradiated with 60 Coγ ray at 3, 6, 9 and 12 Gy respectively to establish the irradiated model. The changes in ALP activity, content of intracellular calcium, and expressions of BMP and TGF β 1 were investigated. Results All the above mentioned indices were significantly decreased after irradiation. There was a close relationship between the enhanced impairment of ALP activity and the increase of irradiation dosages. Conclusion The radiation injury of the osteoblasts is mainly due to the impairment of ALP activity, inhibition of synthesis of growth factors such as BMP and TGF β etc , and interference of calcium as second messenger. Thus, the regeneration and reconstruction capacity of the bone is decreased.

关 键 词：^60Coγ射线 体外培养 成骨细胞 实验研究 

分 类 号：R818.88[医药卫生—放射医学] R322.71[医药卫生—临床医学]