宫内暴露于可卡因的仔鼠脑功能的可塑性研究  

Plasticity of brain function of prenatal cocaine exposure offsprings

作　　者：任嘉谦[1] 关晓伟[1] 宋君[2] 何威[2] 

机构地区：[1]中国医科大学脑研究所,辽宁沈阳110001 [2]中国医科大学组胚学教研室

出　　处：《卫生毒理学杂志》2002年第3期154-156,共3页Journal of Health Toxicology

基　　金：国家自然科学基金资助项目 (39970 772 )

摘　　要：目的　建立妊娠中晚期接触可卡因的小鼠动物模型 ,探讨脑功能的可塑性现象和机制。方法　测量可卡因处理组与对照组仔代小鼠在青春期和成年期的体重与脑重 ;运用高效液相色谱结合电化学检测器技术 (Highporfor manceliquidchromatography electrochemistry ,HPLC EC)检测各仔鼠各时期脑内多巴胺 (dopamine ,DA)及其代谢产物 3 ,4 双羟苯乙酸 (3 ,4 dihydroxyphenylaceticacid ,DOPAC)、高香草酸 (4 hydroxy 3 menthoxyphenylaceticacid ,HVA)的含量 ,同时检测各时期仔鼠再次接触可卡因后脑内DA、DOPAC和HVA的变化。结果　有可卡因宫内接触史的仔鼠在青春期体重和脑重比对照组仔鼠下降 ,脑内DA及其代谢产物含量增高 ,上述变化在成年期恢复正常。各组仔鼠在生后青春期再次接触可卡因后DA及其代谢产物含量均升高 ,此时可卡因处理组仍比对照组含量高 ;在成年期各组仔鼠再次接触可卡因虽DA及其代谢产物虽也升高 ,但无组间差异。结论　妊娠中晚期暴露于可卡因可以引起仔鼠脑多巴胺能神经系统功能的改变 ,这种改变随脑发育的不断完善而恢复正常 ,即具有可塑性 。Objective To study plasticity of brain function by builing prenatal cocaine exposure murine model.Methods To weight body weight and brain weight of offsprings from cacaine(COC) and saline (SAL) groups;To detect concentration of DA,DOPAC and HVA in offsprings' brain from all groups during adolecence and mature period by HPLC\|EC.Results The body and brain weight and the concentration of DA,DOPAC and HVA of offsprings from COC groups were higher than from SAL group in pups,while these became normal in aldults;The concentration of DA,DOPAC and HVA of offsprings which injected cocaine during pups and aldults evelated both,but there existed significant difference in pups between two groups,not in aldults.Conclusion Prenatal cocaine exposure can change offsprings'brain function,but there exists plasticity mechanism which can recover brain normality.The mechanism is available for both resting and stress states.

关 键 词：可卡因 仔鼠 脑功能 可塑性 研究 多巴胺 妊娠 

分 类 号：R996[医药卫生—毒理学] R114[医药卫生—药学]