牛去抗原松质骨载体的生物相容性研究  

Histocompoatibility of deantigenic bovine cancellous bone with rabbit osteoblasts

作　　者：彭磊[1] 胡蕴玉[1] 王臻[1] 王庆良[1] 孟国林[1] 白建萍[1] 李丹[1] 张志培[1] 

机构地区：[1]西安第四军医大学西京医院全军骨科研究所,710032

出　　处：《实用口腔医学杂志》2002年第5期435-437,共3页Journal of Practical Stomatology

基　　金：国家自然科学基金资助 (编号 39770 749)

摘　　要：目的 :用胶原修饰松质骨载体表面 ,增加其生物相容性。方法 :新鲜牛松质骨经脱脂、H2 O2 浸渍及部分脱钙处理 ,制成去抗原牛松质骨载体 (MBC) ,载体表面经胶原处理后 ,将体外培养的兔骨膜成骨细胞复合在其表面 ,分别于 4d、10d和 2 1d取材 ,行扫描电镜观察 ,比较对照组。结果 :4d后 ,松质骨面及孔内即有了细胞粘附生长 ,2 1d全部长满 ,有胶原纤维形成 ;对照组细胞没有长满。结论 :以MBC作为载体 。Objective: To prepare surface with collagen, so as to increase the histocompatibility of deantigenic bovine cancellous bone. Methods: Bovine cancellous bone was degreased and macerated by H 2O 2 and decalcified with 0.3 mol/L HCl to form scaffold, surface of which was treaed with collagen, then cultured rabbit osteoblasts were seeded into the scaffold and cultured in vitro . 4, 10, and 21 days later, the cells on the composite materials were observed under sanning election microscope. Results: On day 4, the cells grew in the surface of the scaffold; on day 21, the cells grow into the pores and on all of the surace of the scaffold.Conclusion: Deantigenic bovine cancellous bone with surfaces treated by collagen is histocompatable with rabbit osteoblasts.

关 键 词：去抗原异种骨载体 成骨细胞 胶原 生物相容性 研究 

分 类 号：R318.0[医药卫生—生物医学工程]