薄层扫描法测定益元口服液中黄芪甲苷的含量  

Determination of Astragaloside Ⅳ in yiyuan oral by TLC Scanning

作　　者：梁爱君[1] 刘云[1] 刘文尧[1] 魏萍[1] 

机构地区：[1]中国人民解放军总后勤部卫生部药品仪器检验所,北京100071

出　　处：《解放军药学学报》2002年第5期312-312,共1页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的　建立薄层扫描法 ,测定益元口服液中黄芪甲苷的含量。方法　自制硅胶G薄层板 ,以氯仿 -甲醇 -水 (13∶6∶2 )10℃以下放置过夜的下层溶液为展开剂 ,检测波长为 5 10nm。结果　黄芪甲苷在 0 .6 3～ 3.15 μg范围内 ,呈良好的线性关系 ,平均回收率为 98.2 1% ,RSD为 1.0 %。结论　本法用于益元口服液中黄芪甲苷的含量测定 。Aim To establish a quantitative determination method by TLC for astragaloside Ⅳ in yiyuan oral.Methods Using silica gel G as the coating substance and chloroform-methanol-water(13∶6∶2,lower layer) as the mobile phase,detecting at 510nm.Results The spots of astragaloside Ⅳ on the TLC plate had a good linearity between its contents and area within the range of 0.63～3.15μg (r=0.995 8).The average recovery was 98.21%,and the RSD was 1.0%.Conclusion This qualitative determination for astragaloside Ⅳ in yiyuan oral is convenient and reliable.

关 键 词：薄层扫描法 益元口服液 黄芪甲苷 含量测定 

分 类 号：R286.0[医药卫生—中药学]