耐盐基因转化蓝藻Synechococcus sp.PCC 7942研究  

STUDIES ON TRANSFORMATION OF SALT-TOLERANT-RELATED GENES TO CYANOBACTERIUM Synechococcus sp. PCC 7942

作　　者：刘广发[1] 张会永[1] 楼士林[1] 周克夫[1] 

机构地区：[1]厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室,361005

出　　处：《海洋科学》2002年第10期43-46,共4页Marine Sciences

基　　金：厦门市科委科技发展计划资助项目350222000104号。

摘　　要：将从极端耐盐的假单胞杆菌中克隆得到的3个基因 ,即转录调控因子基因、NADH脱氢酶Ⅱ基因和磷酸甘油磷酸酯酶基因转入淡水蓝藻Synechococcussp.PCC7942中 ,使受体藻对NaCl的耐受性从低于2.5%显著提高到4.5% ,进一步证明了这3个基因确实与生物的耐盐性密切相关。本实验为进一步培育耐盐经济作物奠定了基础。Three salt-tolerant-related genes, transcriptional regulator gene, NADH dehydrogenase II gene and phosphoglycolate phosphatase gene,which are cloned from Pseudomonas xiamenensis, a species of halophilic bacterium, have been transferred as a gene cluster to cyanobacterium Synechococcus sp. PCC 7942. As a result, the NaCl resistant level of the microorganism has been obviously raised from less than 2.5 % to 4.5 %. It shows that the three genes are close correlative to the ability of salt-resistance. This experiment provides a potential possibility for cultivation of salt-resistant economic plants.

关 键 词：耐盐基因转化 转基因蓝藻 高耐盐性 

分 类 号：Q949.22[生物学—植物学] Q943.2