斜纹夜蛾核多角体病毒抑制苜蓿丫纹夜蛾核多角体病毒诱导的斜纹夜蛾细胞凋亡  

Spodoptera litura Multicapsid Nucleopolyhedrovirus Blocks Autographa californica Multicapsid Nucleopolyhedrovirus-induced Spodoptera litura Cell Apoptosis

作　　者：张萍[1] 杨凯[1] 代小江[1] 庞义[1] 苏德明[2] 

机构地区：[1]中山大学生物防治国家重点实验室,广州510275 [2]复旦大学生命科学学院病毒学研究室,上海200433

出　　处：《生物化学与生物物理进展》2002年第5期702-707,共6页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目 (3 973 0 0 3 0 ;3 980 0 0 92 )~~

摘　　要：野生型苜蓿丫纹夜蛾核多角体病毒 (Autographacalifornicamulticapsidnucleopolyhedrovirus,AcMNPV)感染斜纹夜蛾 (Spodopteralitura)细胞系Sl zsu 1,可引起典型的细胞凋亡 ;但可以在草地夜蛾 (Spodopterafrugiperda)细胞Sf 9中复制并形成多角体 .比较了AcMNPV p35基因在病毒感染两种细胞的复制和转录情况 ,认为 p35在非受纳细胞中及时有效的表达能阻止细胞发生凋亡 ;共感染实验结果表明 ,斜纹夜蛾核多角体病毒(Spodopteralituramulticapsidnucleopolyhedrovirus,SpltMNPV)可以抑制AcMNPV诱导的细胞凋亡并可帮助病毒进行复制 ,推测SpltMNPV基因组中与 p35同源的 p4Wild-type AcMNPV (Autographa california multicapsid nucleopolyhedrovirus) induced Spodoptera litura (Sl-zsu-1) cells apoptosis at 10 similar to 12 h post infection, but it could undergo productive replication in Spodoptera frugiperda (Sf-9) cells. The AcMNPV anti-apoptotic gene, p35 gene replication and transcription in different cell tines (Sl-zsu-1 and Sf-9) were investigated by Southern dot blot and RT-PCR respectively. Differences for p35 gene to synthesize and transcribe in these two cell lines suggested that it could not significantly function in the non-permissive cell tine due to its untimely and insufficient expression. Inoculation of Sl-zsu-1 cells with wild-type SpltMNPV (Spodoptera litura multicapsid nucleopolyhedrovirus) 12 h prior to AcMNPV infection blocked the apoptosis and rescued the AcMNPV productive replication. It is deduced that P49 protein, a P35 homolog encoded by p49 gene in SpltMNPV genome, might accumulate a certain level at 12 h post infection enough to counteract the caspases effect.

关 键 词：斜纹夜蛾核多角体病毒 抑制 苜蓿丫纹夜蛾核多角体病毒 诱导 斜纹夜蛾细胞凋亡 P35基因 

分 类 号：S476.13[农业科学—农业昆虫与害虫防治] Q939.4[农业科学—植物保护]