红莲型细胞质雄性不育水稻线粒体atp6基因转录本的编辑位点研究  

Study on The Editing Sites in The Transcript of atp6 Gene of HL-Rice Mitochondria

作　　者：易平[1] 汪莉[1] 孙清萍[1] 朱英国[1] 

机构地区：[1]武汉大学生命科学学院,植物发育生物学教育部重点实验室,武汉430072

出　　处：《生物化学与生物物理进展》2002年第5期729-733,共5页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目 (3 0 70 5 68)~~

摘　　要：以红莲 (HL)型水稻细胞质雄性不育系A、保持系B及杂种一代F1为材料 ,首次比较研究了红莲型水稻线粒体atp6基因转录本的编辑位点及各位点的编辑频率 .结果表明atp6基因的转录本有 18个编辑位点 ,其中有 15个发生在密码子的第一和第二位点上 ,这些位点的编辑最终会导致氨基酸种类的变化 .18个编辑位点在A、B和F1中没有差异 ,但各位点的编辑频率在引入了核恢复基因的条件下发生了较大的变化 ,完全编辑的比例增加 .这些结果首次证明HL型细胞质雄性不育与线粒体atp6转录本的编辑有一定相关性 。RNA editing is a process in which the genetic information of a gene transcript is changed during or after transcription. RNA editing exists extensively in the higher plant mitochondria, and is a necessary step for forming functional proteins. There may be some relationship between RNA editing and cytoplasmic male sterility (CMS), a kind of phenomenon that is closely associated with mitochondrial genome mutations. The research materials were the gametopthyte male sterility line (A), maintainer line (B) and F-1 hybrid (F-1) of HL-type CMS. cDNAs and DNAs of atp6 were obtained from A, B and F-1 by PCR and RT-PCR. Then sequences of cDNAs and DNAs are compared: A, B and F-1 share the same 15 editing sites found in the transcripts of atp6. The restorer gene in F-1 greatly changed the editing frequency of each editing site. So it is suggested that HL-type CMS is associated with RNA editing of atp6.

关 键 词：红莲型细胞质雄性不育 水稻 线粒体 atp6基因转录本 编辑位点 

分 类 号：S511[农业科学—作物学] Q943[生物学—植物学]