休眠期和营养期包囊游仆虫的纤毛器骨架及其微管蛋白  

Ciliature Cytoskeleton and Microtubulin of Resting Cyst and Vegetative Cell in Euplotes encysticus

作　　者：田沁[1] 张莉[1] 隋淑光[1] 倪兵[1] 顾福康[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《Zoological Research》2002年第5期405-408,T004,共5页动物学研究（英文）

基　　金：国家自然科学基金资助项目 ( 396 70 0 90 )

摘　　要：应用光镜和透射电镜术 ,显示了包囊游仆虫休眠细胞中纤毛器骨架的形态 ,并对该纤毛虫休眠细胞和营养细胞的纤毛器及其α、β -微管蛋白进行了免疫荧光定位的比较研究。由免疫荧光显微术显示 ,包囊游仆虫形成休眠包囊后 ,背部毛基体完整地按原有模式保存下来 ;纤毛杆解聚后微管蛋白多集中在细胞皮层 ,小部分均匀散布在细胞质中。据所得结果认为 ,包囊游仆虫形成包囊后 ,微管蛋白主要有 3个去向 ,即 :①处于自噬泡内被逐步消化 ;②以“微管蛋白库”的形式分布于细胞皮层及细胞质中 ;③保留在残留的基体中。此外 ,以往研究中发现的棘毛基部纤维网络未被标记上 。By using optical and transmission electron microscopy,the orientation pattern of ciliature cytoskeleton during resting period in Euplotes encysticus was demonstrated,and the location of α- and β-tubulin in cells under two different physiological states was compared by immunofluorescence.In the resting cyst,nearly all of ciliary shafts were degenerated;kinetosomes of adoral zone of membranelles and ventral cirri were contracted into cytoplasm;part of kinetosomes of fronto-ventral cirri were disintegrated and disappeared;microtubule layers were preserved beneath the pellicle.It was showed by immunofluorescence that the kinetosomes of dorsal cilia were fully preserved according to the original pattern;after encystment,a slight portion of microtubulin scattered in cytoplasm.On the basis of these experimental results,three destinies of the microtubulin in Euplotes encysticus have been proposed:①be located and gradually digested within autophagic vacuole,②be located in cortex and cytoplasm in a form of 'microtubulin storehouse',or ③be preserved in remained kinetosomes.Moreover,the cortical cirri-base-associated fiber found in the past research was not marked in this experiment,which implied that the fiber system does not belong to microtubule system.

关 键 词：体眠期 营养期 包裹游仆虫 纤毛器骨架 微管蛋白 

分 类 号：Q959.117[生物学—动物学]