转Xa21基因水稻中T-DNA整合的遗传定位  

Genetic Mapping of T-DNA Integration Sites in Xa21 Transgenic Rice

作　　者：朱雪峰[1] 陈学伟[1] 李晓兵[1] 钱前[2] 黄大年[2] 朱立煌[1] 翟文学[1] 

机构地区：[1]中国科学院遗传与发育生物学研究所,北京100101 [2]中国水稻研究所,杭州310006

出　　处：《Acta Genetica Sinica》2002年第10期880-886,共7页

基　　金：国家 8 63高技术项目 (No .10 1 0 1 0 2 0 1);国家转基因植物专项 (No .J99 B 0 0 6)资助~~

摘　　要：利用转抗白叶枯病基因Xa2 1的水稻材料 ,通过TAIL PCR方法扩增T DNA整合的侧翼序列。从中筛选属于水稻基因组DNA的T DNA整合的侧翼序列作为探针 ,将外源基因整合位点定位到窄叶青 /京系 17DH群体构建的水稻分子连锁图谱上。共获得属于水稻基因组DNA的T DNA侧翼序列 2 2个 ,其中的 19个序列在定位群体的两个亲本之间显示RFLP多态性 ,分别定位在水稻基因组的第 3,4 ,5 ,7,9,10 ,11和 12染色体上。带有转基因Xa2 1的T DNA整合的定位为研究外源基因在不同染色体位点的位置效应和稳定遗传打下基础。The transformation mediated by Agrobacterium has been successfully applied to rice in recent years. In the previous research we have transferred the Xa21 gene into five rice varieties of China, using Agrobacterium mediated trasformation. In this study, T DNA flanking sequences of Xa21 transgenic rice lines were obtained by using thermal asymmetric interlaced PCR (TAIL PCR). The flanking sequences which are actual rice DNA were identified and located on molecular linkage map developed from a ZYQ8/JX17 double haploid (DH) population. A total of 22 T DNA flanking rice sequences were isolated. Nineteen of them displayed RFLPs between the two parents, ZYQ8 and JX17, and were mapped on the rice chromosomes, 3, 4, 7, 9, 10, 11 and 12, respectively. The genetic mapping of T DNA integration sites in Xa21 transgenic rice will benefit the study of position effect and stable inheritance of the transgene Xa21.

关 键 词：转Xa21基因水稻 T-DNA整合 遗传定位转基因水稻 XA21 整合位点 

分 类 号：S511[农业科学—作物学]