转Bt抗虫棉各器官毒蛋白的含量及表达  

Content and Expression of Bacillus thuringiensis Insecticidal Protein in Transgenic Cotton

作　　者：王保民[1] 李召虎[1] 李斌[1] 田晓莉[1] 段留生[1] 翟志席[1] 何钟佩[1] 

机构地区：[1]中国农业大学作物化学控制中心,北京100094

出　　处：《农业生物技术学报》2002年第3期215-219,共5页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(39870482)。

摘　　要：以转Bt毒蛋白基因抗虫棉(GossypiumhirsutumL.)中棉所30为实验材料,用ELISA(酶联免疫吸附测定法)测定植株Bt毒蛋白含量,研究在大田栽培条件下中棉所30不同器官中Bt毒蛋白含量及时空变化.结果表明:转Bt基因棉不同器官中的Bt毒蛋白含量存在着明显差异,叶片中的Bt毒蛋白含量远远高于蕾、花、铃,而蕾、花、铃中的含量差异不大.主茎叶片中(07-19标记)Bt毒蛋白含量在叶片展开后0～28d随叶龄增大而升高,28～48 d含量比较稳定.果枝叶中Bt毒蛋白含量的变化规律与主茎叶基本相同,种子和铃壳在花后20 d时Bt毒蛋白含量最低,前期和后期差别较小.在不同生育阶段,相同发育期的叶、蕾、花、铃毒蛋白的表达并未出现明显的前期高、后期低或者峰谷现象.The content of Bacillus thuringiensis(Bt) insecticidal protein(ICP) in transgenic cotton(Gossypium hirsutum L.)CCRI (China Cotton Research Institute)30 was quantitated using Enzyme-linked Immunosorbent Assay(ELISA) under field condition. The results showed that the expression pattern demonstrated temporal and spatial characteristics. The Bt ICP content in leaf was much higher than that in square, bud and boll. But there were no significant difference among the laters. The Bt ICP of axial leaf (marked on 19 July) gradually increased during 0~28 d, kept stable during 28~48 d. The expression pattern in sympodial leaf was the same as that in axial leaf. The Bt ICP contents of seeds and boll wall were the lowest on the twentieth day after flowering, but there was little difference between early and later developing stage of them. In the whole growing season, the content of Bt ICP in the same organ of the same developing stage nearly had no difference.

关 键 词：转BT抗虫棉 器官 毒蛋白 表达 转基因棉花 

分 类 号：S562[农业科学—作物学]