山羊IL-2基因cDNA的克隆、鉴定及序列分析  

Cloning、Identification and Sequence Analysis of the Caprine Interleukin-2 (IL-2)Gene

作　　者：应琦华[1] 李祥瑞[1] 陆承平[1] 

机构地区：[1]南京农业大学农业部动物疫病诊断与免疫重点开放实验室,南京210095

出　　处：《农业生物技术学报》2002年第3期250-254,共5页Journal of Agricultural Biotechnology

摘　　要：根据GenBank发表的山羊(Caprahircus)IL-2基因序列,设计了一对引物。以ConA刺激的外周血淋巴细胞为材料,用RT-PCR的方法,从总RNA中扩增出山羊IL-2基因。琼脂糖电泳显示扩增片段约为500bp。分离纯化片段,克隆入表达质粒pBV220,经酶切鉴定,测序结果显示,克隆的山羊IL-2基因与GenBank上发表的序列一致。该序列与绵羊(Ovisaries )和牛(Bastaurus )的IL-2基因序列同源性最大,在96%以上;氨基酸和抗原性比较分析认为,山羊IL-2与绵羊及牛的IL-2在这两方面有较大的同源性。Based on the published nucleotide sequence of caprine (Capra hircus ) IL-2 gene, a pair of RT-PCR primers were designed and synthesized .Total caprine cell RNA, isolated from ConA-stimulated peripheral blood of caprine, was used as template to generate complementary DNA by reverse transcription.The 500 bp DNA fragments were amplified by polymerase chain reaction (PCR),and cloned into the pBV220 expression vector. DNA sequencing confirmed the fragment was caprine IL-2 and the sequence was identical to that published in GenBank; Sequence analysis showed that the cloned sequence had very high similarity with that of ovine (Ovis aries ) and bovine (Bas taurus) IL-2, over 96%; Comparative analysis of both amino acid sequence and antigenicity showed that the IL-2 of caprine have high similarity to the one of ovine and bovine.

关 键 词：山羊 IL-2基因 CDNA 基因克隆 鉴定 序列分析 

分 类 号：S827[农业科学—畜牧学]