利用基因芯片技术筛选HIV-1F亚型基因限制性显示探针  

Screening of HIV-1 F Subtype Gene Probes Amplified by Restriction Display with Genechip Technology

作　　者：李凌[1] 马文丽[1] 祝骥[1] 石嵘[1] 郑文岭[2] 

机构地区：[1]第一军医大学分子生物学研究所,生物芯片全军重点实验室,广州510515 [2]广州总医院分子肿瘤学研究所,广州510010

出　　处：《中国生物化学与分子生物学报》2002年第5期564-567,共4页Chinese Journal of Biochemistry and Molecular Biology

基　　金：广东省自然科学基金资助 (No .9840 92 );广州市重点科技攻关项目(No .990 4480 2 2 )~~

摘　　要：为筛选限制性显示技术制备的HIV 1F亚型基因探针 ,应用基因芯片打印仪将其有序地打印在玻片上制备基因芯片 .在随机引物延伸的过程中进行HIV样品的荧光标记 ,然后与芯片进行杂交 .杂交后清洗玻片并干燥 ,对芯片进行扫描 ,分析各探针的杂交信号 .从中筛选了 14个基因片段作为芯片下一步研究的探针 .实验证明 。In order to screen the HIV-1 F subtype gene probes amplified by restriction display, the genechips were prepared by printing the gene fragments onto the slides with printer(PixSys 5500). The HIV samples were labelled with fluorescent dye during the random priming and then hybridized with the microarray. After hybridization the chips were cleaned, dried and scanned. And the signal from each probe spot was analyzed. Fourteen gene fragments were suitable for the probes of DNA microarray. It proves that restriction display is an effective method for the preparation of genechip probes.

关 键 词：基因芯片技术 筛选 HIV-1F亚型基因 限制性显示探针 DNA微阵 

分 类 号：R373.9[医药卫生—病原生物学] R446.5[医药卫生—基础医学]