小牛胸腺新胸腺活性因子(TAF-Ⅰ)的纯化和鉴定  

Purification and Characterization of Thymus Activity Factor Ⅰ from Calf Thymus

作　　者：刘希成[1] 刘征[2] 马红[2] 梁荣[2] 张彦明[1] 郑昌学[2] 段明星[2] 

机构地区：[1]西北农林科技大学畜牧兽医学院,杨凌712100 [2]清华大学生物科学与技术系,北京100084

出　　处：《中国生物化学与分子生物学报》2002年第5期619-623,共5页Chinese Journal of Biochemistry and Molecular Biology

摘　　要：以小牛胸腺为原料 ,通过匀浆、冻融、超滤、SephadexG 15凝胶过滤、DEAE SephadexA 2 5阴离子交换层析和反相高效液相色谱等步骤 ,分离得到了胸腺活性因子Ⅰ (thymusactivityfactorⅠ ,TAF Ⅰ ) .5 0 0g小牛胸腺组织中纯化得到了 0 92mg胸腺活性因子Ⅰ .经ESI MS质谱鉴定 ,结果显示其分子量为 6 18 8.氨基酸组成分析显示它由Ala、Gly、Glu、Gln、Lys、Ser 6种氨基酸残基组成 .体外生物学活性实验证明 ,TAF Ⅰ能显著促进人外周血淋巴细胞的EA new thymus activity factorⅠ(TAF-Ⅰ) was isolated and purified from calf thymus by ultrafiltration, Sephadex G-15 gel filtration, DEAE-Sephadex A-25 ion-exchange chromatography and reverse-phase HPLC. 0.92 mg TAF-Ⅰwas obtained from 500g calf thymus tissue. TAF-Ⅰ exhibited a molecular mass of 618.8 by ESI-MS. Amino acid composition analysis showed that this peptide consisted of alanine, glycine, glutamic acid, glutamine, lysine and serine residues. The results of biological activity showed that TAF-Ⅰ specifically increased E-rosette formation of human peripheral blood lymphocytes and increased the proliferative respond of the mitogen ConA-induced mice splenocytes.

关 键 词：小牛胸腺 新胸腺活性因子 TAF-I 纯化 鉴定 

分 类 号：Q517[生物学—生物化学] R392.11[医药卫生—免疫学]