双酚-A和17β-雌二醇对人乳腺癌细胞生长的影响  

Influences of bisphenol-A and 17β-estradiol on the growth of human breast cancer cell (MCF-7).

作　　者：马春艳[1] 郭丽丽 梁前进[1] 李艳平[1] 李素文[1] 王子健[2] 

机构地区：[1]北京师范大学生命科学学院,北京100875 [2]中国科学院生态环境研究中心环境水化学国家重点实验室,北京100085

出　　处：《中国环境科学》2002年第5期408-411,共4页China Environmental Science

基　　金：北京市自然科学基金(8982012); 中国科学院方向项目(KZCX2-414)

摘　　要：通过MTT－细胞增殖活性测定法和双参数流式细胞光度术研究了双酚－A和17β－雌二醇对人乳腺癌细胞株MCF－7增殖、细胞周期以及核酸和蛋白合成的影响。结果表明，在3×10^-16-3×10^-8mol/L范围内，双酚－A和17β－雌二醇都能促进细胞增殖；在3×10^-14mol/L时，细胞增殖活性达到最高值，当作用浓度为3×10^-14mol/L时，细胞周期的S期比例增加，细胞总蛋白表达量明显增加，统计结果表明，双酚－A和17β－雌二醇对MCF－7细胞生长特性的雌激素效应非常相似。The influences of bisphenol-A and 17b-estradiol on human breast cancer cell MCF-7 proliferation and the cell cycle, as well as synthesis of protein and DNA were studied with MTT-cell proliferation activation measurement and double-parameter-flow cytometry. The results showed that the cell proliferation was enhanced in the range of 310-16~310-8 mol/L; and the activity of the cell proliferation reached the maximum at 310-14mol/L, for either bisphenol-A or17b-estradiol. At the level of 310-14mol/L, the S-phase of the cell cycle was increased and the gross protein expression was significantly stimulated. Statistic results showed that bisphenol-A and 17b-estradiol had very similar estrogenic effect on MCF-7 cell growth characteristics.

关 键 词：人 乳腺癌细胞 影响 环境内分泌干扰物 双酚-A 17Β-雌二醇 细胞生长 

分 类 号：R12[医药卫生—环境卫生学]